tetano
Editor, Senior Moderator
STAR Protoc
. 2023 Sep 20;4(4):102584.
doi: 10.1016/j.xpro.2023.102584. Online ahead of print. FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
Camilla Tiezzi[SUP] 1 [/SUP], Marzia Rossi[SUP] 1 [/SUP], Andrea Vecchi[SUP] 2 [/SUP], Sara Doselli[SUP] 2 [/SUP], Amalia Penna[SUP] 2 [/SUP], Paola Fisicaro[SUP] 2 [/SUP], Ilaria Montali[SUP] 1 [/SUP], Camilla Ceccatelli Berti[SUP] 1 [/SUP], Valentina Reverberi[SUP] 2 [/SUP], Anna Montali[SUP] 1 [/SUP], Gabriele Missale[SUP] 3 [/SUP], Carlo Ferrari[SUP] 3 [/SUP], Carolina Boni[SUP] 4 [/SUP]
Affiliations
Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).[SUP]1[/SUP].
Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Immunology.
. 2023 Sep 20;4(4):102584.
doi: 10.1016/j.xpro.2023.102584. Online ahead of print. FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
Camilla Tiezzi[SUP] 1 [/SUP], Marzia Rossi[SUP] 1 [/SUP], Andrea Vecchi[SUP] 2 [/SUP], Sara Doselli[SUP] 2 [/SUP], Amalia Penna[SUP] 2 [/SUP], Paola Fisicaro[SUP] 2 [/SUP], Ilaria Montali[SUP] 1 [/SUP], Camilla Ceccatelli Berti[SUP] 1 [/SUP], Valentina Reverberi[SUP] 2 [/SUP], Anna Montali[SUP] 1 [/SUP], Gabriele Missale[SUP] 3 [/SUP], Carlo Ferrari[SUP] 3 [/SUP], Carolina Boni[SUP] 4 [/SUP]
Affiliations
- PMID: 37733600
- DOI: 10.1016/j.xpro.2023.102584
Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).[SUP]1[/SUP].
Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Immunology.