tetano
Editor, Senior Moderator
J Virol. 2011 Sep 14. [Epub ahead of print]
Stabilisation of influenza virus replication intermediates is dependent on the RNA-binding but not the homo-oligomerisation activity of the viral nucleoprotein.
Vreede FT, Ng AK, Shaw PC, Fodor E.
Source
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom.
Abstract
The influenza virus nucleoprotein (NP) is believed to play a central role in directing a switch from RNA genome transcription to replication by the viral RNA polymerase. However, this role has recently been disputed with the proposal of alternative regulatory mechanisms. It has been suggested that expression of viral polymerase and NP allows genome replication by stabilisation of cRNA replication intermediates and complementary ribonucleoprotein (cRNP) assembly. Here we demonstrate that the RNA-binding activity of NP is necessary for stabilisation of cRNA whereas, surprisingly, homo-oligomerisation of NP is not essential. However, both RNA-binding and homo-oligomerisation activities are essential for genome replication.
PMID:
21917965
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21917965
Stabilisation of influenza virus replication intermediates is dependent on the RNA-binding but not the homo-oligomerisation activity of the viral nucleoprotein.
Vreede FT, Ng AK, Shaw PC, Fodor E.
Source
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom.
Abstract
The influenza virus nucleoprotein (NP) is believed to play a central role in directing a switch from RNA genome transcription to replication by the viral RNA polymerase. However, this role has recently been disputed with the proposal of alternative regulatory mechanisms. It has been suggested that expression of viral polymerase and NP allows genome replication by stabilisation of cRNA replication intermediates and complementary ribonucleoprotein (cRNP) assembly. Here we demonstrate that the RNA-binding activity of NP is necessary for stabilisation of cRNA whereas, surprisingly, homo-oligomerisation of NP is not essential. However, both RNA-binding and homo-oligomerisation activities are essential for genome replication.
PMID:
21917965
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21917965