Specific antibody response of mice after immunization with COS-7 ...

[tropical]

From http://www.birdflubreakingnews.com/

02:26 PM, 10/03/07 Specific antibody response of mice after immunization with COS-7 ... - Journal of Immune Based Therapies and Vaccines - Latest articles

PDF:
http://www.jibtherapies.com/content/pdf/1476-8518-5-10.pdf

 

[gsgs]

Re: Specific antibody response of mice after immunization with COS-7 ...

hmm, I don't want to read it all...

what are COS-7 ?
Is this a new method to produce vaccine not in eggs ?

 

[tropical]

Re: Specific antibody response of mice after immunization with COS-7 ...

hmm, I don't want to read it all...

what are COS-7 ?
Is this a new method to produce vaccine not in eggs ?

COS-7 cell derived avian influenza virus (H5N1) recombinant proteins
______
Original research
Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins

Navin Horthongkham , Tananun Srihtrakul , Niracha Athipanyasilp , Sontana Siritantikorn , Wannee Kantakamalakul , Yong Poovorawan and Ruengpung Sutthent


Journal of Immune Based Therapies and Vaccines 2007, 5:10doi:10.1186/1476-8518-5-10
<TABLE cellSpacing=0 cellPadding=0><TBODY><TR><TD><TR><TD>Published:</TD><TD>3 October 2007</TD></TR></TD></TR></TBODY></TABLE>
Abstract (provisional)

To develop avian influenza H5N1 recombinant protein, the hemagglutinin (HA), neuraminidase (NA), matrix (M), and non-structural (NS1) of avian influenza H5N1 isolates from Thailand were engineered to be expressed in prokaryotic (E. coli) and mammalian cell (COS-7) system. The plasmid pBAD-His and pSec-His were used as vectors for these inserted genes. Mice immunized with purified recombinant proteins at concentration 50-250 ug intramuscularly with Alum adjuvant at week 0, week 2, and week 3 showed a good immunogenicity measured by ELISA and neutralization assay. The HA and NS recombinant proteins produced in COS-7 cells can induce specific antibody titer detected by neutralization assay significantly higher than corresponding recombinant proteins produced in E.coli system. The antibody produced in immunized mice could neutralize heterologous avian influenza virus determined by micro-neutralization assay. This study shows that avian influenza virus H5N1 recombinant proteins produced in mammalian cell system were able to induce neutralizing antibody response.

 

[gsgs]

Re: Specific antibody response of mice after immunization with COS-7 ...

produced in COS-7 inside the host ?

 

[tropical]

Re: Specific antibody response of mice after immunization with COS-7 ...

produced in COS-7 inside the host ?

... engineered to be expressed in prokaryotic (E. coli) and mammalian cell (COS-7) system ...The HA and NS recombinant proteins produced in COS-7 cells can induce specific antibody titer detected by neutralization assay significantly higher than corresponding recombinant proteins produced in E.coli system.

From full text PDF:
To construct the mammalian expression vector, ..., and NS1 protein expression in COS-7 cell line, ... DNA was used for transformation into ... E.Coli cells and ..., ... DNA to transform COS-7 cell line by using polyfect transfection system (Qiagen, USA) - from page 6 PDF full text;
...
Imunization of mice - page 7,8
were immunized three times intramuscularly ... with ... and rM protein produced in E.coli and COS-7 cells plus an emulsion prepared with Alum adjuvant. ...
One week after the last boosting, mice were sacrificed and whole blood was collected for imm. analysis.
Than, ..., and rM protein produced in E.coli and COS-7 cells were selected to immunize 5 groups of mice for each protein ...

Seems selected "from extracted blood"?

hmm, I don't want to read it all...

and that it must be read all...

 

[kent nickell]

Re: Specific antibody response of mice after immunization with COS-7 ...

This is interesting because not only are there the mass production aspects of using cell culture but they also refer to the fact that mammalian cell culture may be better than other types of cell culture in that the antigenic epitopes are modified similarly to that in naturally viral infected human cells. (This could lead to a more immunogenic and protective vaccine) ( http://pecf.epfl.ch/webdav/site/pecf/users/166924/public/wurm.2004.naturebiotech.pdf Wurm, FM Nat Biotechnol 2004)