tetano
Editor, Senior Moderator
J Clin Invest. 2018 Nov 19. pii: 121341. doi: 10.1172/JCI121341. [Epub ahead of print]
[h=1]Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination.[/h] Neu KE[SUP]1,[/SUP][SUP]2[/SUP], Guthmiller JJ[SUP]2[/SUP], Huang M[SUP]2[/SUP], La J[SUP]3[/SUP], Vieira MC[SUP]4[/SUP], Kim K[SUP]4[/SUP], Zheng NY[SUP]2[/SUP], Cortese M[SUP]5[/SUP], Tepora ME[SUP]1[/SUP], Hamel NJ[SUP]1[/SUP], Rojas KT[SUP]2[/SUP], Henry C[SUP]2[/SUP], Shaw D[SUP]1,[/SUP][SUP]2[/SUP], Dulberger CL[SUP]6[/SUP], Pulendran B[SUP]5[/SUP], Cobey S[SUP]4[/SUP], Khan AA[SUP]7[/SUP], Wilson PC[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Spec-seq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity. Our analysis did not find evidence of long-term transcriptional specialization between plasmablasts of different isotypes. However, we did find enhanced transcriptional similarity between clonally related B cells, as well as distinct transcriptional signatures ascribed by BCR vaccine recognition. These data suggest IgG and IgA vaccine-positive plasmablasts are largely similar, whereas IgA vaccine-negative cells appear to be transcriptionally distinct from conventional, terminally differentiated, antigen-induced peripheral blood plasmablasts.
[h=4]KEYWORDS:[/h] Adaptive immunity; B cells; Immunology; Influenza; Vaccines
PMID: 30457979 DOI: 10.1172/JCI121341
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[h=1]Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination.[/h] Neu KE[SUP]1,[/SUP][SUP]2[/SUP], Guthmiller JJ[SUP]2[/SUP], Huang M[SUP]2[/SUP], La J[SUP]3[/SUP], Vieira MC[SUP]4[/SUP], Kim K[SUP]4[/SUP], Zheng NY[SUP]2[/SUP], Cortese M[SUP]5[/SUP], Tepora ME[SUP]1[/SUP], Hamel NJ[SUP]1[/SUP], Rojas KT[SUP]2[/SUP], Henry C[SUP]2[/SUP], Shaw D[SUP]1,[/SUP][SUP]2[/SUP], Dulberger CL[SUP]6[/SUP], Pulendran B[SUP]5[/SUP], Cobey S[SUP]4[/SUP], Khan AA[SUP]7[/SUP], Wilson PC[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Spec-seq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity. Our analysis did not find evidence of long-term transcriptional specialization between plasmablasts of different isotypes. However, we did find enhanced transcriptional similarity between clonally related B cells, as well as distinct transcriptional signatures ascribed by BCR vaccine recognition. These data suggest IgG and IgA vaccine-positive plasmablasts are largely similar, whereas IgA vaccine-negative cells appear to be transcriptionally distinct from conventional, terminally differentiated, antigen-induced peripheral blood plasmablasts.
[h=4]KEYWORDS:[/h] Adaptive immunity; B cells; Immunology; Influenza; Vaccines
PMID: 30457979 DOI: 10.1172/JCI121341
Free full text