tetano
Editor, Senior Moderator
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. 2022 Feb 7;e2107832.
doi: 10.1002/smll.202107832. Online ahead of print.
Plasmonic LAMP: Improving the Detection Specificity and Sensitivity for SARS-CoV-2 by Plasmonic Sensing of Isothermally Amplified Nucleic Acids
Haihang Ye[SUP] 1 [/SUP], Chance Nowak[SUP] 2 3 [/SUP], Yaning Liu[SUP] 1 [/SUP], Yi Li[SUP] 2 4 [/SUP], Tingting Zhang[SUP] 1 [/SUP], Leonidas Bleris[SUP] 2 3 4 [/SUP], Zhenpeng Qin[SUP] 1 4 5 6 [/SUP]
Affiliations
Abstract
The ability to detect pathogens specifically and sensitively is critical to combat infectious diseases outbreaks and pandemics. Colorimetric assays involving loop-mediated isothermal amplification (LAMP) provide simple readouts yet suffer from the intrinsic non-template amplification. Herein, a highly specific and sensitive assay relying on plasmonic sensing of LAMP amplicons via DNA hybridization, termed as plasmonic LAMP, is developed for the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) RNA detection. This work has two important advances. First, gold and silver (Au-Ag) alloy nanoshells are developed as plasmonic sensors that have 4-times stronger extinction in the visible wavelengths and give a 20-times lower detection limit for oligonucleotides over Au counterparts. Second, the integrated method allows cutting the complex LAMP amplicons into short repeats that are amendable for hybridization with oligonucleotide-functionalized Au-Ag nanoshells. In the SARS-CoV-2 RNA detection, plasmonic LAMP takes ≈75 min assay time, achieves a detection limit of 10 copies per reaction, and eliminates the contamination from non-template amplification. It also shows better detection specificity and sensitivity over commercially available LAMP kits due to the additional sequence identification. This work opens a new route for LAMP amplicon detection and provides a method for virus testing at its early representation.
Keywords: SARS-CoV-2; hollow nanostructures; homogeneous assays; loop-mediated isothermal amplification; plasmonics.
. 2022 Feb 7;e2107832.
doi: 10.1002/smll.202107832. Online ahead of print.
Plasmonic LAMP: Improving the Detection Specificity and Sensitivity for SARS-CoV-2 by Plasmonic Sensing of Isothermally Amplified Nucleic Acids
Haihang Ye[SUP] 1 [/SUP], Chance Nowak[SUP] 2 3 [/SUP], Yaning Liu[SUP] 1 [/SUP], Yi Li[SUP] 2 4 [/SUP], Tingting Zhang[SUP] 1 [/SUP], Leonidas Bleris[SUP] 2 3 4 [/SUP], Zhenpeng Qin[SUP] 1 4 5 6 [/SUP]
Affiliations
- PMID: 35129304
- DOI: 10.1002/smll.202107832
Abstract
The ability to detect pathogens specifically and sensitively is critical to combat infectious diseases outbreaks and pandemics. Colorimetric assays involving loop-mediated isothermal amplification (LAMP) provide simple readouts yet suffer from the intrinsic non-template amplification. Herein, a highly specific and sensitive assay relying on plasmonic sensing of LAMP amplicons via DNA hybridization, termed as plasmonic LAMP, is developed for the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) RNA detection. This work has two important advances. First, gold and silver (Au-Ag) alloy nanoshells are developed as plasmonic sensors that have 4-times stronger extinction in the visible wavelengths and give a 20-times lower detection limit for oligonucleotides over Au counterparts. Second, the integrated method allows cutting the complex LAMP amplicons into short repeats that are amendable for hybridization with oligonucleotide-functionalized Au-Ag nanoshells. In the SARS-CoV-2 RNA detection, plasmonic LAMP takes ≈75 min assay time, achieves a detection limit of 10 copies per reaction, and eliminates the contamination from non-template amplification. It also shows better detection specificity and sensitivity over commercially available LAMP kits due to the additional sequence identification. This work opens a new route for LAMP amplicon detection and provides a method for virus testing at its early representation.
Keywords: SARS-CoV-2; hollow nanostructures; homogeneous assays; loop-mediated isothermal amplification; plasmonics.