tetano
Editor, Senior Moderator
ACS Sens. 2019 Nov 5. doi: 10.1021/acssensors.9b01558. [Epub ahead of print] [h=1]Single-Molecule-Based Detection of Conserved Influenza A Virus RNA Promoter Using a Protein Nanopore.[/h]
Oh S[SUP]1,[/SUP][SUP]2[/SUP], Lee MK[SUP]1[/SUP], Chi SW[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 Disease Target Structure Research Center, Division of Biomedical Research , Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 34141 , Republic of Korea. 2 Department of Proteome Structural Biology, KRIBB school of Biosicence , University of Science and Technology , Daejeon 34113 , Republic of Korea.
[h=3]Abstract[/h] Influenza A viruses (IAVs) cause annual epidemic and severe pandemic outbreaks worldwide and result in high mortality. Despite the importance of surveillance for preventing IAV infection, the existing techniques are inefficient for ultrasensitive diagnosis in real time. In this study, we performed protein nanopore-based measurements to detect the highly conserved IAV RNA promoter at the single-molecule level. The binding of specific DNA probes to the IAV RNA promoter generated two types of characteristic nanopore signatures with single or double spikes of current blockade and substantially increased dwell times, which facilitated the discrimination of the IAV promoter from nonspecific macromolecules. Our DNA probe-mediated nanopore sensor will serve as an ultrasensitive, real-time, point-of-care diagnostic tool for highly pathogenic IAVs.
[h=4]KEYWORDS:[/h] DNA probe; Influenza A virus; RNA promoter; biological nanopore; α-hemolysin
PMID: 31689087 DOI: 10.1021/acssensors.9b01558
Oh S[SUP]1,[/SUP][SUP]2[/SUP], Lee MK[SUP]1[/SUP], Chi SW[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 Disease Target Structure Research Center, Division of Biomedical Research , Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 34141 , Republic of Korea. 2 Department of Proteome Structural Biology, KRIBB school of Biosicence , University of Science and Technology , Daejeon 34113 , Republic of Korea.
[h=3]Abstract[/h] Influenza A viruses (IAVs) cause annual epidemic and severe pandemic outbreaks worldwide and result in high mortality. Despite the importance of surveillance for preventing IAV infection, the existing techniques are inefficient for ultrasensitive diagnosis in real time. In this study, we performed protein nanopore-based measurements to detect the highly conserved IAV RNA promoter at the single-molecule level. The binding of specific DNA probes to the IAV RNA promoter generated two types of characteristic nanopore signatures with single or double spikes of current blockade and substantially increased dwell times, which facilitated the discrimination of the IAV promoter from nonspecific macromolecules. Our DNA probe-mediated nanopore sensor will serve as an ultrasensitive, real-time, point-of-care diagnostic tool for highly pathogenic IAVs.
[h=4]KEYWORDS:[/h] DNA probe; Influenza A virus; RNA promoter; biological nanopore; α-hemolysin
PMID: 31689087 DOI: 10.1021/acssensors.9b01558