tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2013 Jan 30. [Epub ahead of print]
Single electroporation delivery of a DNA vaccine coding for the hemagglutinin gene of Asian H5N1 avian influenza generated a protective antibody response in chickens against a North American virus strain.
Ogunremi O, Pasick J, Kobinger GP, Hannaman D, Berhane Y, Clavijo A, van Drunen Littel-van den Hurk S.
Source
Ottawa Laboratory, (Fallowfield), Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, K2H 8P9.
Abstract
Protection against the avian influenza (AI) H5N1 virus is suspected to be mainly conferred by the presence of antibodies directed against the hemagglutinin (HA) protein of the virus. A single electroporation delivery of 100 or 250 μg of a DNA vaccine construct, pCAG-HA, encoding the HA gene of A/Hanoi/30408/2005 (H5N1) in chickens led to the development of anti-HA antibody response in 16 of 17 immunized birds as measured by hemagglutination-inhibition (HI) test, competitive ELISA and an indirect ELISA. Birds vaccinated by electroporation (n = 11) were protected from experimental AI challenge with A/chicken/Pennsylvania/1370/1/1983 (H5N2) as judged by low viral load, no clinical symptoms and no mortality (n = 11). In contrast, only two out of 10 birds vaccinated with the same vaccine dose (100 or 250 μg) but without electroporation developed antibodies. These birds showed high viral load and significant morbidity and mortality after challenge. Seroconversion was reduced in birds electroporated with a low vaccine dose (10 μg) but the antibody positive birds were protected against virus challenge. Non-electroporation delivery of a low dose vaccine did not result in seroconversion and the birds were as susceptible as control groups receiving the control vector. Electroporation delivery of the DNA vaccine led to enhanced antibody responses and to protection against AI virus challenge. The HI test, cELISA or indirect ELISA for anti-H5 antibodies could serve as a good predictor of the potency and efficacy of DNA immunization strategy against AI in chickens.
PMID:
23365205
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23365205
Single electroporation delivery of a DNA vaccine coding for the hemagglutinin gene of Asian H5N1 avian influenza generated a protective antibody response in chickens against a North American virus strain.
Ogunremi O, Pasick J, Kobinger GP, Hannaman D, Berhane Y, Clavijo A, van Drunen Littel-van den Hurk S.
Source
Ottawa Laboratory, (Fallowfield), Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, K2H 8P9.
Abstract
Protection against the avian influenza (AI) H5N1 virus is suspected to be mainly conferred by the presence of antibodies directed against the hemagglutinin (HA) protein of the virus. A single electroporation delivery of 100 or 250 μg of a DNA vaccine construct, pCAG-HA, encoding the HA gene of A/Hanoi/30408/2005 (H5N1) in chickens led to the development of anti-HA antibody response in 16 of 17 immunized birds as measured by hemagglutination-inhibition (HI) test, competitive ELISA and an indirect ELISA. Birds vaccinated by electroporation (n = 11) were protected from experimental AI challenge with A/chicken/Pennsylvania/1370/1/1983 (H5N2) as judged by low viral load, no clinical symptoms and no mortality (n = 11). In contrast, only two out of 10 birds vaccinated with the same vaccine dose (100 or 250 μg) but without electroporation developed antibodies. These birds showed high viral load and significant morbidity and mortality after challenge. Seroconversion was reduced in birds electroporated with a low vaccine dose (10 μg) but the antibody positive birds were protected against virus challenge. Non-electroporation delivery of a low dose vaccine did not result in seroconversion and the birds were as susceptible as control groups receiving the control vector. Electroporation delivery of the DNA vaccine led to enhanced antibody responses and to protection against AI virus challenge. The HI test, cELISA or indirect ELISA for anti-H5 antibodies could serve as a good predictor of the potency and efficacy of DNA immunization strategy against AI in chickens.
PMID:
23365205
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23365205