tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2016 Mar 7. pii: S0732-8893(16)30005-0. doi: 10.1016/j.diagmicrobio.2016.02.004. [Epub ahead of print]
[h=1]Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.[/h] Overmeire Y[SUP]1[/SUP], Vanlaere E[SUP]1[/SUP], Hombrouck A[SUP]2[/SUP], De Beenhouwer H[SUP]3[/SUP], Simons G[SUP]4[/SUP], Brink A[SUP]4[/SUP], Van den Abeele AM[SUP]1[/SUP], Verfaillie C[SUP]1[/SUP], Van Acker J[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic drift; Influenza; Laboratory; Laboratory surveillance; Molecular methods
PMID: 26964723 [PubMed - as supplied by publisher]
[h=1]Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.[/h] Overmeire Y[SUP]1[/SUP], Vanlaere E[SUP]1[/SUP], Hombrouck A[SUP]2[/SUP], De Beenhouwer H[SUP]3[/SUP], Simons G[SUP]4[/SUP], Brink A[SUP]4[/SUP], Van den Abeele AM[SUP]1[/SUP], Verfaillie C[SUP]1[/SUP], Van Acker J[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic drift; Influenza; Laboratory; Laboratory surveillance; Molecular methods
PMID: 26964723 [PubMed - as supplied by publisher]