tetano
Editor, Senior Moderator
PLoS One. 2015 Nov 5;10(11):e0141686. doi: 10.1371/journal.pone.0141686. eCollection 2015.
[h=1]Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.[/h] Huang D[SUP]1[/SUP], Peng WJ[SUP]1[/SUP], Ye Q[SUP]1[/SUP], Liu XP[SUP]1[/SUP], Zhao L[SUP]1[/SUP], Fan L[SUP]1[/SUP], Xia-Hou K[SUP]1[/SUP], Jia HJ[SUP]1[/SUP], Luo J[SUP]2[/SUP], Zhou LT[SUP]2[/SUP], Li BB[SUP]2[/SUP], Wang SL[SUP]2[/SUP], Xu WT[SUP]2[/SUP], Chen Z[SUP]2[/SUP], Tan WS[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51?106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97?106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted.
PMID: 26540170 [PubMed - in process]
[h=1]Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.[/h] Huang D[SUP]1[/SUP], Peng WJ[SUP]1[/SUP], Ye Q[SUP]1[/SUP], Liu XP[SUP]1[/SUP], Zhao L[SUP]1[/SUP], Fan L[SUP]1[/SUP], Xia-Hou K[SUP]1[/SUP], Jia HJ[SUP]1[/SUP], Luo J[SUP]2[/SUP], Zhou LT[SUP]2[/SUP], Li BB[SUP]2[/SUP], Wang SL[SUP]2[/SUP], Xu WT[SUP]2[/SUP], Chen Z[SUP]2[/SUP], Tan WS[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51?106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97?106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted.
PMID: 26540170 [PubMed - in process]