tetano
Editor, Senior Moderator
Mem Inst Oswaldo Cruz. 2011 Aug;106(5):613-6.
Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples.
Ferreira JL, Borborema SE, Br?gido LF, Oliveira MI, Paiva TM, Santos CL.
Source
Centro de Virologia, Instituto Adolfo Lutz, S?o Paulo, SP, Brasil, 01246-000.
Abstract
In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.
PMID:
21894383
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/21894383
Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples.
Ferreira JL, Borborema SE, Br?gido LF, Oliveira MI, Paiva TM, Santos CL.
Source
Centro de Virologia, Instituto Adolfo Lutz, S?o Paulo, SP, Brasil, 01246-000.
Abstract
In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.
PMID:
21894383
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/21894383