tetano
Editor, Senior Moderator
Sens Actuators B Chem
. 2022 Jan 22;131460.
doi: 10.1016/j.snb.2022.131460. Online ahead of print.
Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2
Yuchen Song[SUP] 1 [/SUP], Yuqing Chao[SUP] 2 [/SUP], Yi Guo[SUP] 1 [/SUP], Fan Zhang[SUP] 1 [/SUP], Changqing Mao[SUP] 1 [/SUP], Chaoyang Guan[SUP] 1 [/SUP], Guifang Chen[SUP] 1 [/SUP], Chang Feng[SUP] 1 [/SUP]
Affiliations
Abstract
COVID-19 is a highly diffuse respiratory infection caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Currently, quantitative real-time polymerase chain reaction (qRT-PCR) technology is commonly used in clinical diagnosis of COVID-19. However, this method is time-consuming and labor-intensive, which is limited in clinical application. Here, we propose a new method for the ultrasensitive and visual detection of SARS-CoV-2 viral nucleic acid. The assay integrates with a paper device and highly efficient isothermal amplification technology - Netlike rolling circle amplification (NRCA), which can reach a limit of detection of 4.12 aM. The paper-based NRCA owns advantages of specificity, portability, visualization and low-cost. Therefore, this method can effectively meet the requirements of point-of-care testing, providing a novel molecular detection technology for clinical diagnosis of COVID-19 and promoting the development of NRCA devices.
Keywords: Netlike rolling circle amplification; Paper; Point-of-care testing; SARS-CoV-2.
. 2022 Jan 22;131460.
doi: 10.1016/j.snb.2022.131460. Online ahead of print.
Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2
Yuchen Song[SUP] 1 [/SUP], Yuqing Chao[SUP] 2 [/SUP], Yi Guo[SUP] 1 [/SUP], Fan Zhang[SUP] 1 [/SUP], Changqing Mao[SUP] 1 [/SUP], Chaoyang Guan[SUP] 1 [/SUP], Guifang Chen[SUP] 1 [/SUP], Chang Feng[SUP] 1 [/SUP]
Affiliations
- PMID: 35095201
- PMCID: PMC8782770
- DOI: 10.1016/j.snb.2022.131460
Abstract
COVID-19 is a highly diffuse respiratory infection caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Currently, quantitative real-time polymerase chain reaction (qRT-PCR) technology is commonly used in clinical diagnosis of COVID-19. However, this method is time-consuming and labor-intensive, which is limited in clinical application. Here, we propose a new method for the ultrasensitive and visual detection of SARS-CoV-2 viral nucleic acid. The assay integrates with a paper device and highly efficient isothermal amplification technology - Netlike rolling circle amplification (NRCA), which can reach a limit of detection of 4.12 aM. The paper-based NRCA owns advantages of specificity, portability, visualization and low-cost. Therefore, this method can effectively meet the requirements of point-of-care testing, providing a novel molecular detection technology for clinical diagnosis of COVID-19 and promoting the development of NRCA devices.
Keywords: Netlike rolling circle amplification; Paper; Point-of-care testing; SARS-CoV-2.