tetano
Editor, Senior Moderator
Sens Actuators B Chem
. 2022 Jan 26;131447.
doi: 10.1016/j.snb.2022.131447. Online ahead of print.
An Integrated Microfluidic Platform Featuring Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of COVID-19
You-Ru Jhou[SUP] 1 [/SUP], Chih-Hung Wang[SUP] 1 [/SUP], Huey-Pin Tsai[SUP] 2 3 [/SUP], Yan-Shen Shan[SUP] 4 5 [/SUP], Gwo-Bin Lee[SUP] 1 6 [/SUP]
Affiliations
Abstract
An integrated microfluidic platform (IMP) utilizing real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed here for detection and quantification of three genes of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; i.e., coronavirus diseases 2019 (COVID-19)): RNA-dependent RNA polymerase, the envelope gene, and the nucleocapsid gene for molecular diagnosis. The IMP comprised a microfluidic chip, a temperature control module, a fluidic control module that collectively carried out viral lysis, RNA extraction, RT-LAMP, and the real-time detection within 90 min in an automatic format. A limit of detection of 5×10[SUP]3[/SUP] copies/reaction for each gene was determined with three samples including synthesized RNAs, inactive viruses, and RNAs extracted from clinical samples; this compact platform could be a useful tool for COVID-19 diagnostics.
Keywords: COVID-19; SARS-CoV-2; loop-mediated isothermal amplification; microfluidics; molecular diagnostics.
. 2022 Jan 26;131447.
doi: 10.1016/j.snb.2022.131447. Online ahead of print.
An Integrated Microfluidic Platform Featuring Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of COVID-19
You-Ru Jhou[SUP] 1 [/SUP], Chih-Hung Wang[SUP] 1 [/SUP], Huey-Pin Tsai[SUP] 2 3 [/SUP], Yan-Shen Shan[SUP] 4 5 [/SUP], Gwo-Bin Lee[SUP] 1 6 [/SUP]
Affiliations
- PMID: 35095200
- PMCID: PMC8789398
- DOI: 10.1016/j.snb.2022.131447
Abstract
An integrated microfluidic platform (IMP) utilizing real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed here for detection and quantification of three genes of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; i.e., coronavirus diseases 2019 (COVID-19)): RNA-dependent RNA polymerase, the envelope gene, and the nucleocapsid gene for molecular diagnosis. The IMP comprised a microfluidic chip, a temperature control module, a fluidic control module that collectively carried out viral lysis, RNA extraction, RT-LAMP, and the real-time detection within 90 min in an automatic format. A limit of detection of 5×10[SUP]3[/SUP] copies/reaction for each gene was determined with three samples including synthesized RNAs, inactive viruses, and RNAs extracted from clinical samples; this compact platform could be a useful tool for COVID-19 diagnostics.
Keywords: COVID-19; SARS-CoV-2; loop-mediated isothermal amplification; microfluidics; molecular diagnostics.