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Science DOI: 10.1126/science.1228172
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The Structure of Native Influenza Virion Ribonucleoproteins
Roc?o Arranz1,
Roc?o Coloma2,3,
Francisco Javier Chich?n1,
Jos? Javier Conesa1,
Jos? L. Carrascosa1,4,
Jos? M. Valpuesta1,
Juan Ort?n2,3,*,
Jaime Mart?n-Benito1,*
+ Author Affiliations
1Department of Macromolecular Structure, Centro Nacional de Biotecnolog?a (CSIC), Madrid, Spain.
2Department of Molecular and Cellular Biology, Centro Nacional de Biotecnolog?a (CSIC), Madrid, Spain.
3CIBER de Enfermedades Respiratorias (ISCIII), Madrid, Spain.
4Instituto Madrile?o de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Madrid, Spain.
↵*To whom correspondence should be addressed. E-mail: jmartinb@cnb.csic.es (J.M.-B.); jortin@cnb.csic.es (J.O.)
Abstract
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public health issue. The segmented negative-stranded RNAs are associated with the polymerase complex and nucleoprotein (NP), forming ribonucleoproteins (RNPs), which are responsible for virus transcription and replication. We describe the structure of native RNPs derived from virions. They show a double-helical conformation in which two NP strands of opposite polarity are associated with each other along the helix. Both strands are connected by a short loop at one end of the particle and interact with the polymerase complex at the other end. This structure will be relevant for unraveling the mechanisms of nuclear import of parental virus RNPs, their transcription and replication, and the encapsidation of progeny RNPs into virions.
http://www.sciencemag.org/content/early/2012/11/20/science.1228172
Report
The Structure of Native Influenza Virion Ribonucleoproteins
Roc?o Arranz1,
Roc?o Coloma2,3,
Francisco Javier Chich?n1,
Jos? Javier Conesa1,
Jos? L. Carrascosa1,4,
Jos? M. Valpuesta1,
Juan Ort?n2,3,*,
Jaime Mart?n-Benito1,*
+ Author Affiliations
1Department of Macromolecular Structure, Centro Nacional de Biotecnolog?a (CSIC), Madrid, Spain.
2Department of Molecular and Cellular Biology, Centro Nacional de Biotecnolog?a (CSIC), Madrid, Spain.
3CIBER de Enfermedades Respiratorias (ISCIII), Madrid, Spain.
4Instituto Madrile?o de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Madrid, Spain.
↵*To whom correspondence should be addressed. E-mail: jmartinb@cnb.csic.es (J.M.-B.); jortin@cnb.csic.es (J.O.)
Abstract
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public health issue. The segmented negative-stranded RNAs are associated with the polymerase complex and nucleoprotein (NP), forming ribonucleoproteins (RNPs), which are responsible for virus transcription and replication. We describe the structure of native RNPs derived from virions. They show a double-helical conformation in which two NP strands of opposite polarity are associated with each other along the helix. Both strands are connected by a short loop at one end of the particle and interact with the polymerase complex at the other end. This structure will be relevant for unraveling the mechanisms of nuclear import of parental virus RNPs, their transcription and replication, and the encapsidation of progeny RNPs into virions.
http://www.sciencemag.org/content/early/2012/11/20/science.1228172