Giuseppe
Emeritus
[Source: Science, full text: (LINK). Abstract, edited.]
<CITE><ABBR>Science</ABBR> 23 September 2011: Vol. 333 no. 6050 pp. 1764-1767 - DOI: 10.1126/science.1208747 </CITE>
<CITE></CITE> Acceleration of Emergence of Bacterial Antibiotic Resistance in Connected Microenvironments
Qiucen Zhang<SUP>1</SUP>, Guillaume Lambert<SUP>1</SUP>, David Liao<SUP>2</SUP>, Hyunsung Kim<SUP>3</SUP>, Kristelle Robin<SUP>4</SUP>, Chih-kuan Tung<SUP>5</SUP>, Nader Pourmand<SUP>3</SUP>, Robert H. Austin<SUP>1</SUP>,<SUP>4</SUP>,*
Author Affiliations: <SUP>1</SUP>Department of Physics, Princeton University, Princeton, NJ 08544, USA. <SUP>2</SUP>Department of Pathology, University of California, San Francisco, CA 94143, USA. <SUP>3</SUP>University of California, Santa Cruz Genome Sequencing Center, University of California, Santa Cruz, CA 95064, USA. <SUP>4</SUP>Institute for Advanced Study, Hong Kong University of Science and Technology, Kowloon, Hong Kong. <SUP>5</SUP>Department of Physics, University of Pittsburgh, Pittsburgh, PA 15260, USA.
*To whom correspondence should be addressed. E-mail: austin@princeton.edu
Abstract
The emergence of bacterial antibiotic resistance is a growing problem, yet the variables that influence the rate of emergence of resistance are not well understood. In a microfluidic device designed to mimic naturally occurring bacterial niches, resistance of Escherichia coli to the antibiotic ciprofloxacin developed within 10 hours. Resistance emerged with as few as 100 bacteria in the initial inoculation. Whole-genome sequencing of the resistant organisms revealed that four functional single-nucleotide polymorphisms attained fixation. Knowledge about the rapid emergence of antibiotic resistance in the heterogeneous conditions within the mammalian body may be helpful in understanding the emergence of drug resistance during cancer chemotherapy.
-
------
<CITE><ABBR>Science</ABBR> 23 September 2011: Vol. 333 no. 6050 pp. 1764-1767 - DOI: 10.1126/science.1208747 </CITE>
<CITE></CITE> Acceleration of Emergence of Bacterial Antibiotic Resistance in Connected Microenvironments
Qiucen Zhang<SUP>1</SUP>, Guillaume Lambert<SUP>1</SUP>, David Liao<SUP>2</SUP>, Hyunsung Kim<SUP>3</SUP>, Kristelle Robin<SUP>4</SUP>, Chih-kuan Tung<SUP>5</SUP>, Nader Pourmand<SUP>3</SUP>, Robert H. Austin<SUP>1</SUP>,<SUP>4</SUP>,*
Author Affiliations: <SUP>1</SUP>Department of Physics, Princeton University, Princeton, NJ 08544, USA. <SUP>2</SUP>Department of Pathology, University of California, San Francisco, CA 94143, USA. <SUP>3</SUP>University of California, Santa Cruz Genome Sequencing Center, University of California, Santa Cruz, CA 95064, USA. <SUP>4</SUP>Institute for Advanced Study, Hong Kong University of Science and Technology, Kowloon, Hong Kong. <SUP>5</SUP>Department of Physics, University of Pittsburgh, Pittsburgh, PA 15260, USA.
*To whom correspondence should be addressed. E-mail: austin@princeton.edu
Abstract
The emergence of bacterial antibiotic resistance is a growing problem, yet the variables that influence the rate of emergence of resistance are not well understood. In a microfluidic device designed to mimic naturally occurring bacterial niches, resistance of Escherichia coli to the antibiotic ciprofloxacin developed within 10 hours. Resistance emerged with as few as 100 bacteria in the initial inoculation. Whole-genome sequencing of the resistant organisms revealed that four functional single-nucleotide polymorphisms attained fixation. Knowledge about the rapid emergence of antibiotic resistance in the heterogeneous conditions within the mammalian body may be helpful in understanding the emergence of drug resistance during cancer chemotherapy.
- Received for publication 23 May 2011.
- Accepted for publication 29 July 2011.
------