tetano
Editor, Senior Moderator
Sci Transl Med
. 2021 Jul 20;eabg1143.
doi: 10.1126/scitranslmed.abg1143. Online ahead of print.
A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
Yangtao Wu[SUP] #[/SUP][SUP] 1 [/SUP], Xiaofen Huang[SUP] #[/SUP][SUP] 2 1 [/SUP], Lunzhi Yuan[SUP] #[/SUP][SUP] 1 [/SUP], Shaojuan Wang[SUP] #[/SUP][SUP] 1 [/SUP], Yali Zhang[SUP] #[/SUP][SUP] 1 [/SUP], Hualong Xiong[SUP] #[/SUP][SUP] 2 1 [/SUP], Rirong Chen[SUP] 3 4 [/SUP], Jian Ma[SUP] 2 1 [/SUP], Ruoyao Qi[SUP] 2 1 [/SUP], Meifeng Nie[SUP] 1 [/SUP], Jingjing Xu[SUP] 1 [/SUP], Zhigang Zhang[SUP] 1 1 [/SUP], Liqiang Chen[SUP] 3 4 [/SUP], Min Wei[SUP] 2 1 [/SUP], Ming Zhou[SUP] 2 1 [/SUP], Minping Cai[SUP] 3 4 [/SUP], Yang Shi[SUP] 1 [/SUP], Liang Zhang[SUP] 1 [/SUP], Huan Yu[SUP] 3 4 [/SUP], Junping Hong[SUP] 1 [/SUP], Zikang Wang[SUP] 1 [/SUP], Yunda Hong[SUP] 1 [/SUP], Mingxi Yue[SUP] 1 [/SUP], Zonglin Li[SUP] 1 [/SUP], Dabing Chen[SUP] 1 [/SUP], Qingbing Zheng[SUP] 2 1 [/SUP], Shaowei Li[SUP] 1 [/SUP], Yixin Chen[SUP] 2 1 [/SUP], Tong Cheng[SUP] 1 [/SUP], Jun Zhang[SUP] 5 [/SUP], Tianying Zhang[SUP] 6 1 [/SUP], Huachen Zhu[SUP] 7 3 4 [/SUP], Qinjian Zhao[SUP] 6 1 [/SUP], Quan Yuan[SUP] 5 [/SUP], Yi Guan[SUP] 8 4 [/SUP], Ningshao Xia[SUP] 6 1 2 [/SUP]
Affiliations
Abstract
Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine comprised of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate-modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both Th1- and Th2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit-based SARS-CoV-2 vaccine candidate.
. 2021 Jul 20;eabg1143.
doi: 10.1126/scitranslmed.abg1143. Online ahead of print.
A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
Yangtao Wu[SUP] #[/SUP][SUP] 1 [/SUP], Xiaofen Huang[SUP] #[/SUP][SUP] 2 1 [/SUP], Lunzhi Yuan[SUP] #[/SUP][SUP] 1 [/SUP], Shaojuan Wang[SUP] #[/SUP][SUP] 1 [/SUP], Yali Zhang[SUP] #[/SUP][SUP] 1 [/SUP], Hualong Xiong[SUP] #[/SUP][SUP] 2 1 [/SUP], Rirong Chen[SUP] 3 4 [/SUP], Jian Ma[SUP] 2 1 [/SUP], Ruoyao Qi[SUP] 2 1 [/SUP], Meifeng Nie[SUP] 1 [/SUP], Jingjing Xu[SUP] 1 [/SUP], Zhigang Zhang[SUP] 1 1 [/SUP], Liqiang Chen[SUP] 3 4 [/SUP], Min Wei[SUP] 2 1 [/SUP], Ming Zhou[SUP] 2 1 [/SUP], Minping Cai[SUP] 3 4 [/SUP], Yang Shi[SUP] 1 [/SUP], Liang Zhang[SUP] 1 [/SUP], Huan Yu[SUP] 3 4 [/SUP], Junping Hong[SUP] 1 [/SUP], Zikang Wang[SUP] 1 [/SUP], Yunda Hong[SUP] 1 [/SUP], Mingxi Yue[SUP] 1 [/SUP], Zonglin Li[SUP] 1 [/SUP], Dabing Chen[SUP] 1 [/SUP], Qingbing Zheng[SUP] 2 1 [/SUP], Shaowei Li[SUP] 1 [/SUP], Yixin Chen[SUP] 2 1 [/SUP], Tong Cheng[SUP] 1 [/SUP], Jun Zhang[SUP] 5 [/SUP], Tianying Zhang[SUP] 6 1 [/SUP], Huachen Zhu[SUP] 7 3 4 [/SUP], Qinjian Zhao[SUP] 6 1 [/SUP], Quan Yuan[SUP] 5 [/SUP], Yi Guan[SUP] 8 4 [/SUP], Ningshao Xia[SUP] 6 1 2 [/SUP]
Affiliations
- PMID: 34285130
- DOI: 10.1126/scitranslmed.abg1143
Abstract
Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine comprised of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate-modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both Th1- and Th2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit-based SARS-CoV-2 vaccine candidate.