tetano
Editor, Senior Moderator
Sci Total Environ
. 2020 Nov 10;143578.
doi: 10.1016/j.scitotenv.2020.143578. Online ahead of print.
Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak
Akihiko Hata[SUP] 1 [/SUP], Hiroe Hara-Yamamura[SUP] 2 [/SUP], Yuno Meuchi[SUP] 1 [/SUP], Shota Imai[SUP] 1 [/SUP], Ryo Honda[SUP] 3 [/SUP]
Affiliations
Abstract
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater samples has been documented in several countries. Wastewater-based epidemiology (WBE) is potentially effective for early warning of a COVID-19 outbreak. In this study, presence of SARS-CoV-2 RNA in wastewater samples was investigated and was compared with the number of the confirmed COVID-19 cases in the study area during COVID-19 outbreak in Japan. In total, 45 influent wastewater samples were collected from five wastewater treatment plants in Ishikawa and Toyama prefectures in Japan. During the study period, the numbers of confirmed COVID-19 cases in these prefectures increased from 0.3 and 0 to >20 per 100,000 people. SARS-CoV-2 ribonucleic acid (RNA) in the samples was detected using several PCR-based assays. Of the 45 samples, 21 were positive for SARS-CoV-2 according to at least one of the three quantitative RT-PCR assays. The detection frequency increased when the number of total confirmed SARS-CoV-2 cases in 100,000 people exceeded 10 in each prefecture; however, SARS-CoV-2 could also be detected at a low frequency even when the number was below 1.0. SARS-CoV-2 in wastewater could be detected in the early stage of the epidemic, even if the number of confirmed cases potentially underestimates the actual numbers of cases. This suggests that WBE approach can potentially act as an early warning of COVID-19 outbreaks in Japan.
Keywords: Coronavirus infectious disease 2019 (COVID-19); PEG precipitation; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Wastewater-based epidemiology (WBE); qRT-PCR.
. 2020 Nov 10;143578.
doi: 10.1016/j.scitotenv.2020.143578. Online ahead of print.
Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak
Akihiko Hata[SUP] 1 [/SUP], Hiroe Hara-Yamamura[SUP] 2 [/SUP], Yuno Meuchi[SUP] 1 [/SUP], Shota Imai[SUP] 1 [/SUP], Ryo Honda[SUP] 3 [/SUP]
Affiliations
- PMID: 33221007
- DOI: 10.1016/j.scitotenv.2020.143578
Abstract
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater samples has been documented in several countries. Wastewater-based epidemiology (WBE) is potentially effective for early warning of a COVID-19 outbreak. In this study, presence of SARS-CoV-2 RNA in wastewater samples was investigated and was compared with the number of the confirmed COVID-19 cases in the study area during COVID-19 outbreak in Japan. In total, 45 influent wastewater samples were collected from five wastewater treatment plants in Ishikawa and Toyama prefectures in Japan. During the study period, the numbers of confirmed COVID-19 cases in these prefectures increased from 0.3 and 0 to >20 per 100,000 people. SARS-CoV-2 ribonucleic acid (RNA) in the samples was detected using several PCR-based assays. Of the 45 samples, 21 were positive for SARS-CoV-2 according to at least one of the three quantitative RT-PCR assays. The detection frequency increased when the number of total confirmed SARS-CoV-2 cases in 100,000 people exceeded 10 in each prefecture; however, SARS-CoV-2 could also be detected at a low frequency even when the number was below 1.0. SARS-CoV-2 in wastewater could be detected in the early stage of the epidemic, even if the number of confirmed cases potentially underestimates the actual numbers of cases. This suggests that WBE approach can potentially act as an early warning of COVID-19 outbreaks in Japan.
Keywords: Coronavirus infectious disease 2019 (COVID-19); PEG precipitation; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Wastewater-based epidemiology (WBE); qRT-PCR.