tetano
Editor, Senior Moderator
Sci Rep
. 2023 Aug 4;13(1):12648.
doi: 10.1038/s41598-023-39439-1. Ultrafast inactivation of SARS-CoV-2 by 254-nm UV-C irradiation on porous and non-porous media of medical interest using an omnidirectional chamber
Bertrand Maubert[SUP] #[/SUP][SUP] 1 [/SUP], Camille Theillière[SUP] #[/SUP][SUP] 2 3 [/SUP], Prescillia Jany[SUP] 2 [/SUP], Thomas Bourlet[SUP] 3 4 [/SUP], Jérôme Deschamps[SUP] 5 [/SUP], Bruno Pozzetto[SUP] 3 4 [/SUP], Fateh Singh[SUP] 5 [/SUP], Emilie Gadea[SUP] 6 7 [/SUP]
Affiliations
Covid-19 has spurred a renewed interest in decontamination techniques for air, objects and surfaces. Beginning in 2020, urgent effort was done to permit the reuse of UV-C for inactivating SARS-CoV-2. However, those studies diverged widely on the dose necessary to reach this goal; until today, the real value of the sensitivity of the virus to a 254-nm illumination is not known precisely. In this study, decontamination was performed in an original UV-C large decontamination chamber (UVCab, ON-LIGHT, France) delivering an omnidirectional irradiation with an average dose of 50 mJ/cm[SUP]2[/SUP] in 60 s. Viral inactivation was checked by both cell culture and PCR test. SARS-CoV-2 was inactivated by UV-C light within 3 s on both porous (disposable gown) and non-porous (stainless steel and apron) surfaces. For the porous surface, an irradiation of 5 min was needed to achieve a completely negative PCR signal. The Z value estimating the sensitivity of SARS-CoV-2 to UV-C in the experimental conditions of our cabinet was shown to be > 0.5820 m[SUP]2[/SUP]/J. These results illustrate the ability of this apparatus to inactivate rapidly and definitively high loads of SARS-CoV-2 deposited on porous or non-porous supports and opens new perspectives on material decontamination using UV-C.
. 2023 Aug 4;13(1):12648.
doi: 10.1038/s41598-023-39439-1. Ultrafast inactivation of SARS-CoV-2 by 254-nm UV-C irradiation on porous and non-porous media of medical interest using an omnidirectional chamber
Bertrand Maubert[SUP] #[/SUP][SUP] 1 [/SUP], Camille Theillière[SUP] #[/SUP][SUP] 2 3 [/SUP], Prescillia Jany[SUP] 2 [/SUP], Thomas Bourlet[SUP] 3 4 [/SUP], Jérôme Deschamps[SUP] 5 [/SUP], Bruno Pozzetto[SUP] 3 4 [/SUP], Fateh Singh[SUP] 5 [/SUP], Emilie Gadea[SUP] 6 7 [/SUP]
Affiliations
- PMID: 37542073
- PMCID: PMC10403608
- DOI: 10.1038/s41598-023-39439-1
Covid-19 has spurred a renewed interest in decontamination techniques for air, objects and surfaces. Beginning in 2020, urgent effort was done to permit the reuse of UV-C for inactivating SARS-CoV-2. However, those studies diverged widely on the dose necessary to reach this goal; until today, the real value of the sensitivity of the virus to a 254-nm illumination is not known precisely. In this study, decontamination was performed in an original UV-C large decontamination chamber (UVCab, ON-LIGHT, France) delivering an omnidirectional irradiation with an average dose of 50 mJ/cm[SUP]2[/SUP] in 60 s. Viral inactivation was checked by both cell culture and PCR test. SARS-CoV-2 was inactivated by UV-C light within 3 s on both porous (disposable gown) and non-porous (stainless steel and apron) surfaces. For the porous surface, an irradiation of 5 min was needed to achieve a completely negative PCR signal. The Z value estimating the sensitivity of SARS-CoV-2 to UV-C in the experimental conditions of our cabinet was shown to be > 0.5820 m[SUP]2[/SUP]/J. These results illustrate the ability of this apparatus to inactivate rapidly and definitively high loads of SARS-CoV-2 deposited on porous or non-porous supports and opens new perspectives on material decontamination using UV-C.