tetano
Editor, Senior Moderator
Sci Rep
. 2024 Feb 29;14(1):4974.
doi: 10.1038/s41598-024-55696-0. Severe COVID-19 and long COVID are associated with high expression of STING, cGAS and IFN-α
Maria Alice Freitas Queiroz[SUP] 1 2 [/SUP], Wandrey Roberto Dos Santos Brito[SUP] 3 4 [/SUP], Keise Adrielle Santos Pereira[SUP] 3 4 [/SUP], Leonn Mendes Soares Pereira[SUP] 3 4 [/SUP], Ednelza da Silva Graça Amoras[SUP] 3 [/SUP], Sandra Souza Lima[SUP] 3 [/SUP], Erika Ferreira Dos Santos[SUP] 4 5 [/SUP], Flávia Póvoa da Costa[SUP] 4 5 [/SUP], Kevin Matheus Lima de Sarges[SUP] 4 5 [/SUP], Marcos Henrique Damasceno Cantanhede[SUP] 4 5 [/SUP], Mioni Thieli Figueiredo Magalhães de Brito[SUP] 5 [/SUP], Andréa Luciana Soares da Silva[SUP] 5 [/SUP], Mauro de Meira Leite[SUP] 5 [/SUP], Maria de Nazaré do Socorro de Almeida Viana[SUP] 4 5 [/SUP], Fabíola Brasil Barbosa Rodrigues[SUP] 4 5 [/SUP], Rosilene da Silva[SUP] 4 5 [/SUP], Giselle Maria Rachid Viana[SUP] 6 [/SUP], Tânia do Socorro Souza Chaves[SUP] 6 7 [/SUP], Adriana de Oliveira Lameira Veríssimo[SUP] 8 [/SUP], Mayara da Silva Carvalho[SUP] 8 [/SUP], Daniele Freitas Henriques[SUP] 9 [/SUP], Carla Pinheiro da Silva[SUP] 9 [/SUP], Juliana Abreu Lima Nunes[SUP] 10 [/SUP], Iran Barros Costa[SUP] 10 11 [/SUP], Izaura Maria Vieira Cayres-Vallinoto[SUP] 3 4 [/SUP], Igor Brasil-Costa[SUP] 10 11 [/SUP], Juarez Antônio Simões Quaresma[SUP] 11 12 [/SUP], Luiz Fábio Magno Falcão[SUP] 12 [/SUP], Eduardo José Melo Dos Santos[SUP] 4 5 [/SUP], Antonio Carlos Rosário Vallinoto[SUP] 3 4 11 [/SUP]
Affiliations
The cGAS-STING pathway appears to contribute to dysregulated inflammation during coronavirus disease 2019 (COVID-19); however, inflammatory factors related to long COVID are still being investigated. In the present study, we evaluated the association of cGAS and STING gene expression levels and plasma IFN-α, TNF-α and IL-6 levels with COVID-19 severity in acute infection and long COVID, based on analysis of blood samples from 148 individuals, 87 with acute COVID-19 and 61 in the post-COVID-19 period. Quantification of gene expression was performed by real-time PCR, and cytokine levels were quantified by ELISA and flow cytometry. In acute COVID-19, cGAS, STING, IFN-α, TNF-α, and IL-6 levels were higher in patients with severe disease than in those with nonsevere manifestations (p < 0.05). Long COVID was associated with elevated cGAS, STING and IFN-α levels (p < 0.05). Activation of the cGAS-STING pathway may contribute to an intense systemic inflammatory state in severe COVID-19 and, after infection resolution, induce an autoinflammatory disease in some tissues, resulting in long COVID.
. 2024 Feb 29;14(1):4974.
doi: 10.1038/s41598-024-55696-0. Severe COVID-19 and long COVID are associated with high expression of STING, cGAS and IFN-α
Maria Alice Freitas Queiroz[SUP] 1 2 [/SUP], Wandrey Roberto Dos Santos Brito[SUP] 3 4 [/SUP], Keise Adrielle Santos Pereira[SUP] 3 4 [/SUP], Leonn Mendes Soares Pereira[SUP] 3 4 [/SUP], Ednelza da Silva Graça Amoras[SUP] 3 [/SUP], Sandra Souza Lima[SUP] 3 [/SUP], Erika Ferreira Dos Santos[SUP] 4 5 [/SUP], Flávia Póvoa da Costa[SUP] 4 5 [/SUP], Kevin Matheus Lima de Sarges[SUP] 4 5 [/SUP], Marcos Henrique Damasceno Cantanhede[SUP] 4 5 [/SUP], Mioni Thieli Figueiredo Magalhães de Brito[SUP] 5 [/SUP], Andréa Luciana Soares da Silva[SUP] 5 [/SUP], Mauro de Meira Leite[SUP] 5 [/SUP], Maria de Nazaré do Socorro de Almeida Viana[SUP] 4 5 [/SUP], Fabíola Brasil Barbosa Rodrigues[SUP] 4 5 [/SUP], Rosilene da Silva[SUP] 4 5 [/SUP], Giselle Maria Rachid Viana[SUP] 6 [/SUP], Tânia do Socorro Souza Chaves[SUP] 6 7 [/SUP], Adriana de Oliveira Lameira Veríssimo[SUP] 8 [/SUP], Mayara da Silva Carvalho[SUP] 8 [/SUP], Daniele Freitas Henriques[SUP] 9 [/SUP], Carla Pinheiro da Silva[SUP] 9 [/SUP], Juliana Abreu Lima Nunes[SUP] 10 [/SUP], Iran Barros Costa[SUP] 10 11 [/SUP], Izaura Maria Vieira Cayres-Vallinoto[SUP] 3 4 [/SUP], Igor Brasil-Costa[SUP] 10 11 [/SUP], Juarez Antônio Simões Quaresma[SUP] 11 12 [/SUP], Luiz Fábio Magno Falcão[SUP] 12 [/SUP], Eduardo José Melo Dos Santos[SUP] 4 5 [/SUP], Antonio Carlos Rosário Vallinoto[SUP] 3 4 11 [/SUP]
Affiliations
- PMID: 38424312
- DOI: 10.1038/s41598-024-55696-0
The cGAS-STING pathway appears to contribute to dysregulated inflammation during coronavirus disease 2019 (COVID-19); however, inflammatory factors related to long COVID are still being investigated. In the present study, we evaluated the association of cGAS and STING gene expression levels and plasma IFN-α, TNF-α and IL-6 levels with COVID-19 severity in acute infection and long COVID, based on analysis of blood samples from 148 individuals, 87 with acute COVID-19 and 61 in the post-COVID-19 period. Quantification of gene expression was performed by real-time PCR, and cytokine levels were quantified by ELISA and flow cytometry. In acute COVID-19, cGAS, STING, IFN-α, TNF-α, and IL-6 levels were higher in patients with severe disease than in those with nonsevere manifestations (p < 0.05). Long COVID was associated with elevated cGAS, STING and IFN-α levels (p < 0.05). Activation of the cGAS-STING pathway may contribute to an intense systemic inflammatory state in severe COVID-19 and, after infection resolution, induce an autoinflammatory disease in some tissues, resulting in long COVID.