tetano
Editor, Senior Moderator
Sci Rep
. 2024 Dec 30;14(1):31799.
doi: 10.1038/s41598-024-82901-x. Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care
Richard Vincent[SUP] 1 [/SUP], David Rapoport[SUP] 2 [/SUP], Priti Balchandani[SUP] #[/SUP][SUP] 3 4 5 [/SUP], Joseph Borrello[SUP] 6 [/SUP], Michael Schotsaert[SUP] 7 8 9 10 [/SUP], Robert Karlicek[SUP] 11 [/SUP], Gabriel Laghlali[SUP] 7 8 [/SUP], Prajakta Warang[SUP] 7 8 [/SUP], Seokchan Park[SUP] 7 8 [/SUP], Gagandeep Singh[SUP] 7 8 [/SUP], Isabella Morgan[SUP] 6 [/SUP], James Paredes[SUP] 12 [/SUP], Raveen Rathnasinghe[SUP] 7 8 13 [/SUP], Jacob Wolf[SUP] 6 [/SUP], Adolfo García-Sastre[SUP] #[/SUP][SUP] 7 8 10 14 15 16 [/SUP]
Affiliations
Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototype portable UV-C[SUB]254[/SUB] device to sterilize high flows of viral-contaminated air from a simulated patient source at airflow rates of up to 100 l/m. Our device consisted of a central quartz tube surrounded 6 high-output UV-C[SUB]254[/SUB] lamps, within a larger cylinder allowing recirculation past the UV-C[SUB]254[/SUB] lamps a second time before exiting the device. Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. Turning on varying numbers of lamps controlled the dose of UVC. Viability experiments at low, medium and high (100 l/min) flows of contaminated gas were conducted with 6, 4, 2 and 1 lamp activated (single-pass and recirculation were tested). Our data show 5-log reduction in plaque forming units from a single lamp (single- pass and recirculated conditions) at high and low flows. UVC dose at 100 l/m was calculated at 11.6 mJ/cm[SUP]2[/SUP] single pass and 104 mJ/cm[SUP]2[/SUP] recirculated. The protype device shows high efficacy in killing nebulized influenza virus in a high flow of contaminated air.
. 2024 Dec 30;14(1):31799.
doi: 10.1038/s41598-024-82901-x. Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care
Richard Vincent[SUP] 1 [/SUP], David Rapoport[SUP] 2 [/SUP], Priti Balchandani[SUP] #[/SUP][SUP] 3 4 5 [/SUP], Joseph Borrello[SUP] 6 [/SUP], Michael Schotsaert[SUP] 7 8 9 10 [/SUP], Robert Karlicek[SUP] 11 [/SUP], Gabriel Laghlali[SUP] 7 8 [/SUP], Prajakta Warang[SUP] 7 8 [/SUP], Seokchan Park[SUP] 7 8 [/SUP], Gagandeep Singh[SUP] 7 8 [/SUP], Isabella Morgan[SUP] 6 [/SUP], James Paredes[SUP] 12 [/SUP], Raveen Rathnasinghe[SUP] 7 8 13 [/SUP], Jacob Wolf[SUP] 6 [/SUP], Adolfo García-Sastre[SUP] #[/SUP][SUP] 7 8 10 14 15 16 [/SUP]
Affiliations
- PMID: 39738586
- PMCID: PMC11686185
- DOI: 10.1038/s41598-024-82901-x
Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototype portable UV-C[SUB]254[/SUB] device to sterilize high flows of viral-contaminated air from a simulated patient source at airflow rates of up to 100 l/m. Our device consisted of a central quartz tube surrounded 6 high-output UV-C[SUB]254[/SUB] lamps, within a larger cylinder allowing recirculation past the UV-C[SUB]254[/SUB] lamps a second time before exiting the device. Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. Turning on varying numbers of lamps controlled the dose of UVC. Viability experiments at low, medium and high (100 l/min) flows of contaminated gas were conducted with 6, 4, 2 and 1 lamp activated (single-pass and recirculation were tested). Our data show 5-log reduction in plaque forming units from a single lamp (single- pass and recirculated conditions) at high and low flows. UVC dose at 100 l/m was calculated at 11.6 mJ/cm[SUP]2[/SUP] single pass and 104 mJ/cm[SUP]2[/SUP] recirculated. The protype device shows high efficacy in killing nebulized influenza virus in a high flow of contaminated air.