tetano
Editor, Senior Moderator
Sci Rep
. 2024 Oct 30;14(1):26057.
doi: 10.1038/s41598-024-77862-0. Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids
Tetsuya Kuwano[SUP] 1 [/SUP], Takayuki Kanno[SUP] 2 [/SUP], Minoru Tobiume[SUP] 2 [/SUP], Yuichiro Hirata[SUP] 2 [/SUP], Harutaka Katano[SUP] 2 [/SUP], Michiko Koga[SUP] 3 4 [/SUP], Hiroyuki Nagai[SUP] 4 [/SUP], Takeya Tsutsumi[SUP] 3 4 [/SUP], Noritada Yoshikawa[SUP] 5 [/SUP], Hiroshi Yotsuyanagi[SUP] 3 4 [/SUP], Satoshi Kutsuna[SUP] 6 7 [/SUP], Yusuke Miyazato[SUP] 6 8 [/SUP], Noriko Kinoshita-Iwamoto[SUP] 6 [/SUP], Norio Ohmagari[SUP] 6 [/SUP], Taiichiro Kobayashi[SUP] 9 [/SUP], Kazuaki Fukushima[SUP] 9 [/SUP], Masaru Tanaka[SUP] 9 [/SUP], Akifumi Imamura[SUP] 9 [/SUP], Yui Ueda[SUP] 10 [/SUP], Maeko Iwamura[SUP] 10 [/SUP], Naoto Takada[SUP] 10 [/SUP], Takayoshi Inoue[SUP] 10 [/SUP], Tetsuro Matano[SUP] 11 12 [/SUP], Ai Kawana-Tachikawa[SUP] 11 12 [/SUP], Tadaki Suzuki[SUP] 13 [/SUP]
Affiliations
There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6-10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19.
Keywords: COVID-19; SARS-CoV-2; Sebum; Skin; Skin surface lipid; Transcriptome.
. 2024 Oct 30;14(1):26057.
doi: 10.1038/s41598-024-77862-0. Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids
Tetsuya Kuwano[SUP] 1 [/SUP], Takayuki Kanno[SUP] 2 [/SUP], Minoru Tobiume[SUP] 2 [/SUP], Yuichiro Hirata[SUP] 2 [/SUP], Harutaka Katano[SUP] 2 [/SUP], Michiko Koga[SUP] 3 4 [/SUP], Hiroyuki Nagai[SUP] 4 [/SUP], Takeya Tsutsumi[SUP] 3 4 [/SUP], Noritada Yoshikawa[SUP] 5 [/SUP], Hiroshi Yotsuyanagi[SUP] 3 4 [/SUP], Satoshi Kutsuna[SUP] 6 7 [/SUP], Yusuke Miyazato[SUP] 6 8 [/SUP], Noriko Kinoshita-Iwamoto[SUP] 6 [/SUP], Norio Ohmagari[SUP] 6 [/SUP], Taiichiro Kobayashi[SUP] 9 [/SUP], Kazuaki Fukushima[SUP] 9 [/SUP], Masaru Tanaka[SUP] 9 [/SUP], Akifumi Imamura[SUP] 9 [/SUP], Yui Ueda[SUP] 10 [/SUP], Maeko Iwamura[SUP] 10 [/SUP], Naoto Takada[SUP] 10 [/SUP], Takayoshi Inoue[SUP] 10 [/SUP], Tetsuro Matano[SUP] 11 12 [/SUP], Ai Kawana-Tachikawa[SUP] 11 12 [/SUP], Tadaki Suzuki[SUP] 13 [/SUP]
Affiliations
- PMID: 39472469
- DOI: 10.1038/s41598-024-77862-0
There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6-10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19.
Keywords: COVID-19; SARS-CoV-2; Sebum; Skin; Skin surface lipid; Transcriptome.