tetano
Editor, Senior Moderator
Sci Rep
. 2024 Nov 16;14(1):28292.
doi: 10.1038/s41598-024-79407-x. Microfluidic qPCR for detection of 21 common respiratory viruses in children with influenza-like illness
Thomas J Saville[SUP] #[/SUP][SUP] 1 2 [/SUP], Hayley Colton[SUP] #[/SUP][SUP] 3 4 5 [/SUP], Sheikh Jarju[SUP] 6 [/SUP], Edwin P Armitage[SUP] 6 7 [/SUP], Sainabou Drammeh[SUP] 6 [/SUP], Simon Tazzyman[SUP] 8 [/SUP], Ya Jankey Jagne[SUP] 6 [/SUP], Hadijatou J Sallah[SUP] 6 [/SUP], Elina Senghore[SUP] 6 [/SUP], Cariad M Evans[SUP] 9 10 8 [/SUP], Thomas C Darton[SUP] 9 10 [/SUP], Thushan I de Silva[SUP] 9 10 6 7 [/SUP]
Affiliations
Multiple respiratory viruses lead to high morbidity and mortality, yet global surveillance platforms focus primarily on seasonal influenza viruses. The COVID-19 pandemic and new RSV vaccines highlight the importance of a broader approach. Upper respiratory tract swabs from children aged 24-59 months presenting with influenza-like illness in The Gambia were collected during follow-up of a live-attenuated influenza vaccine randomised controlled trial in 2017-18. A microfluidic quantitative polymerase chain reaction (qPCR) assay was established and used to detect 21 respiratory viruses. 76.6% of samples had one or more viruses detected (n = 121/158). The viruses detected most frequently were rhinovirus (n = 37/158, 23.4%) and adenovirus (n = 34/158, 21.5%), followed by parainfluenza virus 3, influenza B and human metapneumovirus B. A third of positive samples had multiple viruses detected (two n = 31/121, 25.6%; three n = 9/121, 7.4%). Our data demonstrates how microfluidic qPCR is a useful tool for high-throughput, comprehensive detection of multiple respiratory viruses in surveillance platforms. Rapidly changing epidemiology exemplifies the need for new, broader approaches to virus surveillance in low-resource settings to respond to future epidemics and to guide the need for and use of new prevention and therapeutic measures.
Keywords: Influenza-like illness; Microfluidics; Polymerase chain reaction; Respiratory virus; Surveillance.
. 2024 Nov 16;14(1):28292.
doi: 10.1038/s41598-024-79407-x. Microfluidic qPCR for detection of 21 common respiratory viruses in children with influenza-like illness
Thomas J Saville[SUP] #[/SUP][SUP] 1 2 [/SUP], Hayley Colton[SUP] #[/SUP][SUP] 3 4 5 [/SUP], Sheikh Jarju[SUP] 6 [/SUP], Edwin P Armitage[SUP] 6 7 [/SUP], Sainabou Drammeh[SUP] 6 [/SUP], Simon Tazzyman[SUP] 8 [/SUP], Ya Jankey Jagne[SUP] 6 [/SUP], Hadijatou J Sallah[SUP] 6 [/SUP], Elina Senghore[SUP] 6 [/SUP], Cariad M Evans[SUP] 9 10 8 [/SUP], Thomas C Darton[SUP] 9 10 [/SUP], Thushan I de Silva[SUP] 9 10 6 7 [/SUP]
Affiliations
- PMID: 39550452
- PMCID: PMC11569225
- DOI: 10.1038/s41598-024-79407-x
Multiple respiratory viruses lead to high morbidity and mortality, yet global surveillance platforms focus primarily on seasonal influenza viruses. The COVID-19 pandemic and new RSV vaccines highlight the importance of a broader approach. Upper respiratory tract swabs from children aged 24-59 months presenting with influenza-like illness in The Gambia were collected during follow-up of a live-attenuated influenza vaccine randomised controlled trial in 2017-18. A microfluidic quantitative polymerase chain reaction (qPCR) assay was established and used to detect 21 respiratory viruses. 76.6% of samples had one or more viruses detected (n = 121/158). The viruses detected most frequently were rhinovirus (n = 37/158, 23.4%) and adenovirus (n = 34/158, 21.5%), followed by parainfluenza virus 3, influenza B and human metapneumovirus B. A third of positive samples had multiple viruses detected (two n = 31/121, 25.6%; three n = 9/121, 7.4%). Our data demonstrates how microfluidic qPCR is a useful tool for high-throughput, comprehensive detection of multiple respiratory viruses in surveillance platforms. Rapidly changing epidemiology exemplifies the need for new, broader approaches to virus surveillance in low-resource settings to respond to future epidemics and to guide the need for and use of new prevention and therapeutic measures.
Keywords: Influenza-like illness; Microfluidics; Polymerase chain reaction; Respiratory virus; Surveillance.