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Sci Rep . Inflammatory, fibrotic and endothelial biomarker profiles in COVID-19 patients during and following hospitalization

tetano

Editor, Senior Moderator
Sci Rep


. 2025 Jul 10;15(1):24850.
doi: 10.1038/s41598-025-09245-y. Inflammatory, fibrotic and endothelial biomarker profiles in COVID-19 patients during and following hospitalization

Fares Darawshy[SUP] #[/SUP][SUP] 1 2 [/SUP], Dan Padawer[SUP] #[/SUP][SUP] 1 2 [/SUP], Abed Qadan[SUP] 2 [/SUP], Yaser Salaymeh[SUP] 1 2 [/SUP], Neville Berkman[SUP] 3 4 [/SUP]



Affiliations
Abstract

Survivors of severe COVID-19 often suffer from long-term respiratory issues, but the molecular drivers of this damage remain unclear. This study explored the dynamics of inflammatory, fibrotic, and endothelial biomarkers in hospitalized COVID-19 patients. Plasma levels of MMP2, MMP7, MMP9, KL-6, IL-6, TNF-α, angiopoietin-2, thrombomodulin, and hyaluronan were measured in 73 hospitalized COVID-19 patients during admission and, in a subset, at follow-up. Pulmonary function tests were performed at follow-up. The majority of patients (82.2%) had severe COVID-19; 14 (19.2%) died within 90 days. During acute illness, IL-6, angiopoietin-2, and hyaluronan levels were significantly elevated compared to healthy controls. Follow-up data from 18 patients showed persistent symptoms such as dyspnea and fatigue. MMP2 and MMP7 levels increased at follow-up, while hyaluronan decreased. Angiopoietin-2 remained elevated and was negatively correlated with forced vital capacity (r = - 0.76, p < 0.01). A machine learning approach revealed that an increase in angiopoietin-2 was the most important biomarker associated with FVC variability. Circulating factors associated with fibrosis and endothelial injury remain elevated in COVID-19 survivors and are associated with impaired lung function, indicating that fibrotic and endothelial damage may underlie post-COVID pulmonary sequelae.

Keywords: COVID-19; Endothelial damage; Post-COVID-19; Pulmonary fibrosis.

 
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