tetano
Editor, Senior Moderator
Sci Rep
. 2024 Jun 14;14(1):13702.
doi: 10.1038/s41598-024-64325-9. Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection
Aline de Souza Nicoletti[SUP] 1 [/SUP], Marília Berlofa Visacri[SUP] 2 [/SUP], Carla Regina da Silva Correa da Ronda[SUP] 3 [/SUP], Julia Tiemi Siguemoto[SUP] 1 [/SUP], Carolini Motta Neri[SUP] 3 [/SUP], Rafael Nogueira de Souza[SUP] 1 [/SUP], Deise de Souza Ventura[SUP] 4 [/SUP], Adriana Eguti[SUP] 4 [/SUP], Lilian Ferreira de Souza Silva[SUP] 4 [/SUP], Mauricio Wesley Perroud Junior[SUP] 1 4 [/SUP], Keini Buosi[SUP] 1 [/SUP], Mehrsa Jalalizadeh[SUP] 1 [/SUP], Franciele Dionato[SUP] 1 [/SUP], Luciana Dal Col[SUP] 1 [/SUP], Cristiane Giacomelli[SUP] 1 [/SUP], Patrícia Leme[SUP] 1 [/SUP], Leonardo Oliveira Reis[SUP] 1 5 [/SUP], Luiz Augusto Dos Santos[SUP] 6 [/SUP], Nelson Durán[SUP] 1 [/SUP], Wagner José Fávaro[SUP] 1 [/SUP], José Luiz da Costa[SUP] 3 [/SUP], Carolina Dagli-Hernandez[SUP] 3 [/SUP], Patricia Moriel[SUP] 7 [/SUP], Eder de Carvalho Pincinato[SUP] 1 [/SUP]
Affiliations
Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host-virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mechanisms of the epigenetic regulation of gene expression and play an important role in viral infections. A pilot study published by our research group identified, through next-generation sequencing (NGS), that miR-4433b-5p, miR-320b, and miR-16-2-3p are differentially expressed between patients with COVID-19 and controls. Thus, the objectives of this study were to validate the expression of these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform in silico analyses. Patients with COVID-19 (n = 90) and healthy volunteers (n = 40) were recruited. MiRNAs were extracted from plasma samples and validated using qRT-PCR. In addition, in silico analyses were performed using mirPath v.3 software. MiR-320b was the only miRNA upregulated in the case group com-pared to the control group. The in silico analyses indicated the role of miR-320b in the regulation of the KITLG gene and consequently in the inflammatory process. This study confirmed that miR-320b can distinguish patients with COVID-19 from control participants; however, further research is needed to determine whether this miRNA can be used as a target or a biomarker.
Keywords: COVID-19; Epigenetic; SARS-CoV-2; miRNA; microRNA.
. 2024 Jun 14;14(1):13702.
doi: 10.1038/s41598-024-64325-9. Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection
Aline de Souza Nicoletti[SUP] 1 [/SUP], Marília Berlofa Visacri[SUP] 2 [/SUP], Carla Regina da Silva Correa da Ronda[SUP] 3 [/SUP], Julia Tiemi Siguemoto[SUP] 1 [/SUP], Carolini Motta Neri[SUP] 3 [/SUP], Rafael Nogueira de Souza[SUP] 1 [/SUP], Deise de Souza Ventura[SUP] 4 [/SUP], Adriana Eguti[SUP] 4 [/SUP], Lilian Ferreira de Souza Silva[SUP] 4 [/SUP], Mauricio Wesley Perroud Junior[SUP] 1 4 [/SUP], Keini Buosi[SUP] 1 [/SUP], Mehrsa Jalalizadeh[SUP] 1 [/SUP], Franciele Dionato[SUP] 1 [/SUP], Luciana Dal Col[SUP] 1 [/SUP], Cristiane Giacomelli[SUP] 1 [/SUP], Patrícia Leme[SUP] 1 [/SUP], Leonardo Oliveira Reis[SUP] 1 5 [/SUP], Luiz Augusto Dos Santos[SUP] 6 [/SUP], Nelson Durán[SUP] 1 [/SUP], Wagner José Fávaro[SUP] 1 [/SUP], José Luiz da Costa[SUP] 3 [/SUP], Carolina Dagli-Hernandez[SUP] 3 [/SUP], Patricia Moriel[SUP] 7 [/SUP], Eder de Carvalho Pincinato[SUP] 1 [/SUP]
Affiliations
- PMID: 38871789
- DOI: 10.1038/s41598-024-64325-9
Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host-virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mechanisms of the epigenetic regulation of gene expression and play an important role in viral infections. A pilot study published by our research group identified, through next-generation sequencing (NGS), that miR-4433b-5p, miR-320b, and miR-16-2-3p are differentially expressed between patients with COVID-19 and controls. Thus, the objectives of this study were to validate the expression of these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform in silico analyses. Patients with COVID-19 (n = 90) and healthy volunteers (n = 40) were recruited. MiRNAs were extracted from plasma samples and validated using qRT-PCR. In addition, in silico analyses were performed using mirPath v.3 software. MiR-320b was the only miRNA upregulated in the case group com-pared to the control group. The in silico analyses indicated the role of miR-320b in the regulation of the KITLG gene and consequently in the inflammatory process. This study confirmed that miR-320b can distinguish patients with COVID-19 from control participants; however, further research is needed to determine whether this miRNA can be used as a target or a biomarker.
Keywords: COVID-19; Epigenetic; SARS-CoV-2; miRNA; microRNA.