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Sci Rep . In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods

tetano

Editor, Senior Moderator
Sci Rep


. 2021 Jan 28;11(1):2418.
doi: 10.1038/s41598-021-82148-w.
In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods


Guo Xiling[SUP] #[/SUP][SUP] 1 2 [/SUP], Chen Yin[SUP] #[/SUP][SUP] 1 2 [/SUP], Wang Ling[SUP] 1 [/SUP], Wu Xiaosong[SUP] 1 2 [/SUP], Fan Jingjing[SUP] 1 [/SUP], Li Fang[SUP] 1 [/SUP], Zeng Xiaoyan[SUP] 1 2 [/SUP], Ge Yiyue[SUP] 1 2 [/SUP], Chi Ying[SUP] 1 2 [/SUP], Cui Lunbiao[SUP] 1 2 [/SUP], Zhang Liubo[SUP] 3 [/SUP], Sun Hong[SUP] 4 [/SUP], Xu Yan[SUP] 5 6 [/SUP]



Affiliations

Abstract

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is currently a global pandemic, and there are limited laboratory studies targeting pathogen resistance. This study aimed to investigate the effect of selected disinfection products and methods on the inactivation of SARS-CoV-2 in the laboratory. We used quantitative suspension testing to evaluate the effectiveness of the disinfectant/method. Available chlorine of 250 mg/L, 500 mg/L, and 1000 mg/L required 20 min, 5 min, and 0.5 min to inactivate SARS-CoV-2, respectively. A 600-fold dilution of 17% concentration of di-N-decyl dimethyl ammonium bromide (283 mg/L) and the same concentration of di-N-decyl dimethyl ammonium chloride required only 0.5 min to inactivate the virus efficiently. At 30% concentration for 1 min and 40% and above for 0.5 min, ethanol could efficiently inactivate SARS-CoV-2. Heat takes approximately 30 min at 56 ?C, 10 min above 70 ?C, or 5 min above 90 ?C to inactivate the virus. The chlorinated disinfectants, Di-N-decyl dimethyl ammonium bromide/chloride, ethanol, and heat could effectively inactivate SARS-CoV-2 in the laboratory test. The response of SARS-CoV-2 to disinfectants is very similar to that of SARS-CoV.
 
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