tetano
Editor, Senior Moderator
Sci Rep
. 2024 Sep 9;14(1):20930.
doi: 10.1038/s41598-024-71419-x. Immunologic mediators profile in COVID-19 convalescence
Alexander Leonardo Silva-Junior[SUP] 1 2 [/SUP], Lucas Silva Oliveira[SUP] 1 2 [/SUP], Stephanny Dias[SUP] 2 3 [/SUP], Thaina Cristina Cardoso Costa[SUP] 2 [/SUP], Lilyane Amorim Xabregas[SUP] 2 [/SUP], Fabíola Silva Alves-Hanna[SUP] 2 4 [/SUP], Cláudia Maria Moura Abrahim[SUP] 2 [/SUP], Walter Luiz Lima Neves[SUP] 2 [/SUP], Myuki Alfaia Esashika Crispim[SUP] 2 [/SUP], Diana Mota Toro[SUP] 4 [/SUP], Pedro Vieira Silva-Neto[SUP] 4 [/SUP], Danielle Costa Marques Aponte[SUP] 5 [/SUP], Tatiana Campos Oliveira[SUP] 5 [/SUP], Maria Carmo Costa Silva[SUP] 6 [/SUP], Miharu Maguinoria Matsuura Matos[SUP] 5 [/SUP], Maria Perpétuo Socorro Sampaio Carvalho[SUP] 2 [/SUP], Andrea Monteiro Tarragô[SUP] 2 3 6 [/SUP], Nelson Abrahim Fraiji[SUP] 2 [/SUP], Lúcia Helena Faccioli[SUP] 7 [/SUP], Carlos Artério Sorgi[SUP] 4 8 [/SUP], Ester Cerdeira Sabino[SUP] 9 [/SUP], Andrea Teixeira-Carvalho[SUP] 10 [/SUP], Olindo Assis Martins-Filho[SUP] 10 [/SUP], Allyson Guimarães Costa[SUP] 11 12 13 14 [/SUP], Adriana Malheiro[SUP] 15 16 17 18 19 [/SUP]
Affiliations
SARS-CoV-2 caused the pandemic situation experienced since the beginning of 2020, and many countries faced the rapid spread and severe form of the disease. Mechanisms of interaction between the virus and the host were observed during acute phase, but few data are available when related to immunity dynamics in convalescents. We conducted a longitudinal study, with 51 healthy donors and 62 COVID-19 convalescent patients, which these had a 2-month follow-up after symptoms recovery. Venous blood sample was obtained from all participants to measure blood count, subpopulations of monocytes, lymphocytes, natural killer cells and dendritic cells. Serum was used to measure cytokines, chemokines, growth factors, anti-N IgG and anti-S IgG/IgM antibodies. Statistic was performed by Kruskal-Wallis test, and linear regression with days post symptoms and antibody titers. All analysis had confidence interval of 95%. Less than 35% of convalescents were anti-S IgM+, while more than 80% were IgG+ in D30. Anti-N IgG decreased along time, with loss of seroreactivity of 13%. Eosinophil count played a distinct role on both antibodies during all study, and the convalescence was orchestrated by higher neutrophil-to-lymphocyte ratio and IL-15, but initial stages were marked by increase in myeloid DCs, B1 lymphocytes, inflammatory and patrolling monocytes, G-CSF and IL-2. Later convalescence seemed to change to cytotoxicity mediated by T lymphocytes, plasmacytoid DCs, VEGF, IL-9 and CXCL10. Anti-S IgG antibodies showed the longest perseverance and may be a better option for diagnosis. The inflammatory pattern is yet present on initial stage of convalescence, but quickly shifts to a reparative dynamic. Meanwhile eosinophils seem to play a role on anti-N levels in convalescence, although may not be the major causative agent. We must highlight the importance of immunological markers on acute clinical outcomes, but their comprehension to potentialize adaptive system must be explored to improve immunizations and further preventive policies.
Keywords: Antibody; Brazil; Immune hallmarks; Severe acute respiratory syndrome (SARS).
. 2024 Sep 9;14(1):20930.
doi: 10.1038/s41598-024-71419-x. Immunologic mediators profile in COVID-19 convalescence
Alexander Leonardo Silva-Junior[SUP] 1 2 [/SUP], Lucas Silva Oliveira[SUP] 1 2 [/SUP], Stephanny Dias[SUP] 2 3 [/SUP], Thaina Cristina Cardoso Costa[SUP] 2 [/SUP], Lilyane Amorim Xabregas[SUP] 2 [/SUP], Fabíola Silva Alves-Hanna[SUP] 2 4 [/SUP], Cláudia Maria Moura Abrahim[SUP] 2 [/SUP], Walter Luiz Lima Neves[SUP] 2 [/SUP], Myuki Alfaia Esashika Crispim[SUP] 2 [/SUP], Diana Mota Toro[SUP] 4 [/SUP], Pedro Vieira Silva-Neto[SUP] 4 [/SUP], Danielle Costa Marques Aponte[SUP] 5 [/SUP], Tatiana Campos Oliveira[SUP] 5 [/SUP], Maria Carmo Costa Silva[SUP] 6 [/SUP], Miharu Maguinoria Matsuura Matos[SUP] 5 [/SUP], Maria Perpétuo Socorro Sampaio Carvalho[SUP] 2 [/SUP], Andrea Monteiro Tarragô[SUP] 2 3 6 [/SUP], Nelson Abrahim Fraiji[SUP] 2 [/SUP], Lúcia Helena Faccioli[SUP] 7 [/SUP], Carlos Artério Sorgi[SUP] 4 8 [/SUP], Ester Cerdeira Sabino[SUP] 9 [/SUP], Andrea Teixeira-Carvalho[SUP] 10 [/SUP], Olindo Assis Martins-Filho[SUP] 10 [/SUP], Allyson Guimarães Costa[SUP] 11 12 13 14 [/SUP], Adriana Malheiro[SUP] 15 16 17 18 19 [/SUP]
Affiliations
- PMID: 39251702
- DOI: 10.1038/s41598-024-71419-x
SARS-CoV-2 caused the pandemic situation experienced since the beginning of 2020, and many countries faced the rapid spread and severe form of the disease. Mechanisms of interaction between the virus and the host were observed during acute phase, but few data are available when related to immunity dynamics in convalescents. We conducted a longitudinal study, with 51 healthy donors and 62 COVID-19 convalescent patients, which these had a 2-month follow-up after symptoms recovery. Venous blood sample was obtained from all participants to measure blood count, subpopulations of monocytes, lymphocytes, natural killer cells and dendritic cells. Serum was used to measure cytokines, chemokines, growth factors, anti-N IgG and anti-S IgG/IgM antibodies. Statistic was performed by Kruskal-Wallis test, and linear regression with days post symptoms and antibody titers. All analysis had confidence interval of 95%. Less than 35% of convalescents were anti-S IgM+, while more than 80% were IgG+ in D30. Anti-N IgG decreased along time, with loss of seroreactivity of 13%. Eosinophil count played a distinct role on both antibodies during all study, and the convalescence was orchestrated by higher neutrophil-to-lymphocyte ratio and IL-15, but initial stages were marked by increase in myeloid DCs, B1 lymphocytes, inflammatory and patrolling monocytes, G-CSF and IL-2. Later convalescence seemed to change to cytotoxicity mediated by T lymphocytes, plasmacytoid DCs, VEGF, IL-9 and CXCL10. Anti-S IgG antibodies showed the longest perseverance and may be a better option for diagnosis. The inflammatory pattern is yet present on initial stage of convalescence, but quickly shifts to a reparative dynamic. Meanwhile eosinophils seem to play a role on anti-N levels in convalescence, although may not be the major causative agent. We must highlight the importance of immunological markers on acute clinical outcomes, but their comprehension to potentialize adaptive system must be explored to improve immunizations and further preventive policies.
Keywords: Antibody; Brazil; Immune hallmarks; Severe acute respiratory syndrome (SARS).