tetano
Editor, Senior Moderator
Sci Rep
. 2022 Jun 20;12(1):10340.
doi: 10.1038/s41598-022-13777-y.
Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus
Mitra Gultom[SUP] #[/SUP][SUP] 1 2 3 4 [/SUP], Annika Kratzel[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Jasmine Portmann[SUP] 1 2 [/SUP], Hanspeter Stalder[SUP] 1 2 [/SUP], Astrid Chanfon Bätzner[SUP] 5 [/SUP], Hans Gantenbein[SUP] 2 6 [/SUP], Corinne Gurtner[SUP] 2 6 [/SUP], Nadine Ebert[SUP] 1 2 [/SUP], Hans Henrik Gad[SUP] 7 [/SUP], Rune Hartmann[SUP] 7 [/SUP], Horst Posthaus[SUP] 2 6 [/SUP], Patrik Zanolari[SUP] 8 [/SUP], Stephanie Pfaender[SUP] 1 2 9 [/SUP], Volker Thiel[SUP] 10 11 12 [/SUP], Ronald Dijkman[SUP] 13 14 15 16 [/SUP]
Affiliations
Abstract
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.
. 2022 Jun 20;12(1):10340.
doi: 10.1038/s41598-022-13777-y.
Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus
Mitra Gultom[SUP] #[/SUP][SUP] 1 2 3 4 [/SUP], Annika Kratzel[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Jasmine Portmann[SUP] 1 2 [/SUP], Hanspeter Stalder[SUP] 1 2 [/SUP], Astrid Chanfon Bätzner[SUP] 5 [/SUP], Hans Gantenbein[SUP] 2 6 [/SUP], Corinne Gurtner[SUP] 2 6 [/SUP], Nadine Ebert[SUP] 1 2 [/SUP], Hans Henrik Gad[SUP] 7 [/SUP], Rune Hartmann[SUP] 7 [/SUP], Horst Posthaus[SUP] 2 6 [/SUP], Patrik Zanolari[SUP] 8 [/SUP], Stephanie Pfaender[SUP] 1 2 9 [/SUP], Volker Thiel[SUP] 10 11 12 [/SUP], Ronald Dijkman[SUP] 13 14 15 16 [/SUP]
Affiliations
- PMID: 35725865
- DOI: 10.1038/s41598-022-13777-y
Abstract
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.