tetano
Editor, Senior Moderator
Sci Rep
. 2022 Mar 8;12(1):4080.
doi: 10.1038/s41598-022-08066-7.
Early IFN-β administration protects cigarette smoke exposed mice against lethal influenza virus infection without increasing lung inflammation
Wenxin Wu[SUP] 1 [/SUP], Lili Tian[SUP] 2 [/SUP], Wei Zhang[SUP] 2 [/SUP], J Leland Booth[SUP] 2 [/SUP], Jerry William Ritchey[SUP] 3 [/SUP], Shuhua Wu[SUP] 4 [/SUP], Chao Xu[SUP] 5 [/SUP], Brent R Brown[SUP] 2 [/SUP], Jordan P Metcalf[SUP] 6 7 8 [/SUP]
Affiliations
Abstract
During influenza A virus (IAV) infection, it is unclear whether type I interferons (IFNs) have defensive antiviral effects or contribute to immunopathology in smokers. We treated nonsmoking (NS) and cigarette smoke (CS)-exposed mice intranasally with early (prophylactic) or late (therapeutic) IFN-β. We compared the mortality and innate immune responses of the treated mice following challenge with IAV. In NS mice, both early and late IFN-β administration decreased the survival rate in mice infected with IAV, with late IFN-β administration having the greatest effect on survival. In contrast, in CS-exposed mice, early IFN-β administration significantly increased survival during IAV infection while late IFN-β administration did not alter mortality. With regards to inflammation, in NS mice, IFN-β administration, especially late administration, significantly increased IAV-induced inflammation and lung injury. Early IFN-β administration to CS-exposed mice did not increase IAV-induced inflammation and lung injury as occurred in NS mice. Our results demonstrate, although IFN-β administration worsens the susceptibility of NS mice to influenza infection with increased immunopathology, early IFN-β administration to CS-exposed mice, which have suppression of the intrinsic IFN response, improved outcomes during influenza infection.
. 2022 Mar 8;12(1):4080.
doi: 10.1038/s41598-022-08066-7.
Early IFN-β administration protects cigarette smoke exposed mice against lethal influenza virus infection without increasing lung inflammation
Wenxin Wu[SUP] 1 [/SUP], Lili Tian[SUP] 2 [/SUP], Wei Zhang[SUP] 2 [/SUP], J Leland Booth[SUP] 2 [/SUP], Jerry William Ritchey[SUP] 3 [/SUP], Shuhua Wu[SUP] 4 [/SUP], Chao Xu[SUP] 5 [/SUP], Brent R Brown[SUP] 2 [/SUP], Jordan P Metcalf[SUP] 6 7 8 [/SUP]
Affiliations
- PMID: 35260752
- DOI: 10.1038/s41598-022-08066-7
Abstract
During influenza A virus (IAV) infection, it is unclear whether type I interferons (IFNs) have defensive antiviral effects or contribute to immunopathology in smokers. We treated nonsmoking (NS) and cigarette smoke (CS)-exposed mice intranasally with early (prophylactic) or late (therapeutic) IFN-β. We compared the mortality and innate immune responses of the treated mice following challenge with IAV. In NS mice, both early and late IFN-β administration decreased the survival rate in mice infected with IAV, with late IFN-β administration having the greatest effect on survival. In contrast, in CS-exposed mice, early IFN-β administration significantly increased survival during IAV infection while late IFN-β administration did not alter mortality. With regards to inflammation, in NS mice, IFN-β administration, especially late administration, significantly increased IAV-induced inflammation and lung injury. Early IFN-β administration to CS-exposed mice did not increase IAV-induced inflammation and lung injury as occurred in NS mice. Our results demonstrate, although IFN-β administration worsens the susceptibility of NS mice to influenza infection with increased immunopathology, early IFN-β administration to CS-exposed mice, which have suppression of the intrinsic IFN response, improved outcomes during influenza infection.