tetano
Editor, Senior Moderator
Sci Rep
. 2024 May 27;14(1):12125.
doi: 10.1038/s41598-024-53747-0. Development of a novel Colorimetric Assay for the rapid diagnosis of Coronavirus disease 2019 from nasopharyngeal samples
Neda Sepahi[SUP] 1 [/SUP], Sahar Samsami[SUP] 2 [/SUP], Yaser Mansoori[SUP] 1 [/SUP], Maryam Chenari[SUP] 3 [/SUP], Negin Namavari[SUP] 4 [/SUP], Ava Yazdanpanah[SUP] 2 [/SUP], Abdolmajid Ghasemian[SUP] 1 [/SUP], Zahra Montaseri[SUP] 5 [/SUP], Mahboobeh Sharifzadeh[SUP] 1 [/SUP], Razie Ranjbar[SUP] 1 [/SUP], Sahar Namavari[SUP] 6 [/SUP], Ali Ghanbariasad[SUP] 7 [/SUP]
Affiliations
Emergence of Coronavirus disease 2019 (COVID-19) pandemic has posed a huge threat to public health. Rapid and reliable test to diagnose infected subjects is crucial for disease spread control. We developed a colorimetric test for COVID-19 detection using a Colorimetric Assay based on thiol-linked RNA modified gold nanoparticles (AuNPs) and oligonucleotide probes. This method was conducted on RNA from 200 pharyngeal swab samples initially tested by Real-Time polymerase chain reaction (RT-PCR) as gold standard. A specific oligonucleotide probe designed based on ORF1ab of COVID-19 was functionalized with AuNPs-probe conjugate. The exposure of AuNP-probe to isolated RNA samples was tested using hybridization. In this comparative study, the colorimetric functionalized AuNPs assay exhibited a detection limit of 25 copies/µL. It was higher in comparison to the RT-PCR method, which could only detect 15 copies/µL. The results demonstrated 100% specificity and 96% sensitivity for the developed method. Herein, we developed an incredibly rapid, simple and cost-effective Colorimetric Assay lasting approximately 30 min which could process considerably higher number of COVID-19 samples compared to the RT-PCR. This AuNP-probe conjugate colorimetric method could be considered the optimum alternatives for conventional diagnostic tools especially in over-populated and/or low-income countries.
Keywords: AuNP-probe conjugate; Coronavirus disease 2019; Diagnosis; Real-Time PCR.
. 2024 May 27;14(1):12125.
doi: 10.1038/s41598-024-53747-0. Development of a novel Colorimetric Assay for the rapid diagnosis of Coronavirus disease 2019 from nasopharyngeal samples
Neda Sepahi[SUP] 1 [/SUP], Sahar Samsami[SUP] 2 [/SUP], Yaser Mansoori[SUP] 1 [/SUP], Maryam Chenari[SUP] 3 [/SUP], Negin Namavari[SUP] 4 [/SUP], Ava Yazdanpanah[SUP] 2 [/SUP], Abdolmajid Ghasemian[SUP] 1 [/SUP], Zahra Montaseri[SUP] 5 [/SUP], Mahboobeh Sharifzadeh[SUP] 1 [/SUP], Razie Ranjbar[SUP] 1 [/SUP], Sahar Namavari[SUP] 6 [/SUP], Ali Ghanbariasad[SUP] 7 [/SUP]
Affiliations
- PMID: 38802360
- DOI: 10.1038/s41598-024-53747-0
Emergence of Coronavirus disease 2019 (COVID-19) pandemic has posed a huge threat to public health. Rapid and reliable test to diagnose infected subjects is crucial for disease spread control. We developed a colorimetric test for COVID-19 detection using a Colorimetric Assay based on thiol-linked RNA modified gold nanoparticles (AuNPs) and oligonucleotide probes. This method was conducted on RNA from 200 pharyngeal swab samples initially tested by Real-Time polymerase chain reaction (RT-PCR) as gold standard. A specific oligonucleotide probe designed based on ORF1ab of COVID-19 was functionalized with AuNPs-probe conjugate. The exposure of AuNP-probe to isolated RNA samples was tested using hybridization. In this comparative study, the colorimetric functionalized AuNPs assay exhibited a detection limit of 25 copies/µL. It was higher in comparison to the RT-PCR method, which could only detect 15 copies/µL. The results demonstrated 100% specificity and 96% sensitivity for the developed method. Herein, we developed an incredibly rapid, simple and cost-effective Colorimetric Assay lasting approximately 30 min which could process considerably higher number of COVID-19 samples compared to the RT-PCR. This AuNP-probe conjugate colorimetric method could be considered the optimum alternatives for conventional diagnostic tools especially in over-populated and/or low-income countries.
Keywords: AuNP-probe conjugate; Coronavirus disease 2019; Diagnosis; Real-Time PCR.