tetano
Editor, Senior Moderator
Sci Rep
. 2022 Mar 30;12(1):5418.
doi: 10.1038/s41598-022-09218-5.
Detection and quantification of infectious severe acute respiratory coronavirus-2 in diverse clinical and environmental samples
Yi-Chan Lin[SUP] 1 [/SUP], Rebecca J Malott[SUP] 2 [/SUP], Linda Ward[SUP] 2 3 [/SUP], Linet Kiplagat[SUP] 2 [/SUP], Kanti Pabbaraju[SUP] 4 [/SUP], Kara Gill[SUP] 4 [/SUP], Byron M Berenger[SUP] 2 4 [/SUP], Jia Hu[SUP] 2 [/SUP], Kevin Fonseca[SUP] 2 3 4 [/SUP], Ryan S Noyce[SUP] 1 [/SUP], Thomas Louie[SUP] 2 3 [/SUP], David H Evans[SUP] 5 [/SUP], John M Conly[SUP] 2 3 [/SUP]
Affiliations
Abstract
To explore the potential modes of Severe Acute Respiratory Coronavirus-2 (SARS-CoV-2) transmission, we collected 535 diverse clinical and environmental samples from 75 infected hospitalized and community patients. Infectious SARS-CoV-2 with quantitative burdens varying from 5 plaque-forming units/mL (PFU/mL) up to 1.0 × 10[SUP]6[/SUP] PFU/mL was detected in 151/459 (33%) of the specimens assayed and up to 1.3 × 10[SUP]6[/SUP] PFU/mL on fomites with confirmation by plaque morphology, PCR, immunohistochemistry, and/or sequencing. Infectious virus in clinical and associated environmental samples correlated with time since symptom onset with no detection after 7-8 days in immunocompetent hosts and with N-gene based C[SUB]t[/SUB] values ≤ 25 significantly predictive of yielding plaques in culture. SARS-CoV-2 isolated from patient respiratory tract samples caused illness in a hamster model with a minimum infectious dose of ≤ 14 PFU. Together, our findings offer compelling evidence that large respiratory droplet and contact (direct and indirect i.e., fomites) are important modes of SARS-CoV-2 transmission.
. 2022 Mar 30;12(1):5418.
doi: 10.1038/s41598-022-09218-5.
Detection and quantification of infectious severe acute respiratory coronavirus-2 in diverse clinical and environmental samples
Yi-Chan Lin[SUP] 1 [/SUP], Rebecca J Malott[SUP] 2 [/SUP], Linda Ward[SUP] 2 3 [/SUP], Linet Kiplagat[SUP] 2 [/SUP], Kanti Pabbaraju[SUP] 4 [/SUP], Kara Gill[SUP] 4 [/SUP], Byron M Berenger[SUP] 2 4 [/SUP], Jia Hu[SUP] 2 [/SUP], Kevin Fonseca[SUP] 2 3 4 [/SUP], Ryan S Noyce[SUP] 1 [/SUP], Thomas Louie[SUP] 2 3 [/SUP], David H Evans[SUP] 5 [/SUP], John M Conly[SUP] 2 3 [/SUP]
Affiliations
- PMID: 35354854
- DOI: 10.1038/s41598-022-09218-5
Abstract
To explore the potential modes of Severe Acute Respiratory Coronavirus-2 (SARS-CoV-2) transmission, we collected 535 diverse clinical and environmental samples from 75 infected hospitalized and community patients. Infectious SARS-CoV-2 with quantitative burdens varying from 5 plaque-forming units/mL (PFU/mL) up to 1.0 × 10[SUP]6[/SUP] PFU/mL was detected in 151/459 (33%) of the specimens assayed and up to 1.3 × 10[SUP]6[/SUP] PFU/mL on fomites with confirmation by plaque morphology, PCR, immunohistochemistry, and/or sequencing. Infectious virus in clinical and associated environmental samples correlated with time since symptom onset with no detection after 7-8 days in immunocompetent hosts and with N-gene based C[SUB]t[/SUB] values ≤ 25 significantly predictive of yielding plaques in culture. SARS-CoV-2 isolated from patient respiratory tract samples caused illness in a hamster model with a minimum infectious dose of ≤ 14 PFU. Together, our findings offer compelling evidence that large respiratory droplet and contact (direct and indirect i.e., fomites) are important modes of SARS-CoV-2 transmission.