tetano
Editor, Senior Moderator
Sci Rep
. 2021 Nov 26;11(1):23009.
doi: 10.1038/s41598-021-02128-y.
A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
Erica Frew[SUP] #[/SUP][SUP] 1 [/SUP], Douglas Roberts[SUP] #[/SUP][SUP] 2 [/SUP], Shelly Barry[SUP] 3 [/SUP], Matthew Holden[SUP] 3 [/SUP], Amanda Restell Mand[SUP] 3 [/SUP], Emily Mitsock[SUP] 2 [/SUP], Enqing Tan[SUP] 3 [/SUP], Wei Yu[SUP] 2 [/SUP], Johan Skog[SUP] 2 [/SUP]
Affiliations
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 disease. RT-qPCR has been the primary method of diagnosis; however, the required infrastructure is lacking in many developing countries and the virus has remained a global challenge. More inexpensive and immediate test methods are required to facilitate local, regional, and national management strategies to re-open world economies. Here we have developed a SARS-CoV-2 antigen test in an inexpensive lateral flow format to generate a chromatographic result identifying the presence of the SARS-CoV-2 antigen, and thus an active infection, within a patient anterior nares swab sample. Our 15-min test requires no equipment or laboratory infrastructure to administer with a limit of detection of 2.0 × 10[SUP]2[/SUP] TCID[SUB]50[/SUB]/mL and 87.5% sensitivity, 100% specificity when tested against 40 known positive and 40 known negative patient samples established by a validated RT-qPCR test.
. 2021 Nov 26;11(1):23009.
doi: 10.1038/s41598-021-02128-y.
A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
Erica Frew[SUP] #[/SUP][SUP] 1 [/SUP], Douglas Roberts[SUP] #[/SUP][SUP] 2 [/SUP], Shelly Barry[SUP] 3 [/SUP], Matthew Holden[SUP] 3 [/SUP], Amanda Restell Mand[SUP] 3 [/SUP], Emily Mitsock[SUP] 2 [/SUP], Enqing Tan[SUP] 3 [/SUP], Wei Yu[SUP] 2 [/SUP], Johan Skog[SUP] 2 [/SUP]
Affiliations
- PMID: 34837001
- DOI: 10.1038/s41598-021-02128-y
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 disease. RT-qPCR has been the primary method of diagnosis; however, the required infrastructure is lacking in many developing countries and the virus has remained a global challenge. More inexpensive and immediate test methods are required to facilitate local, regional, and national management strategies to re-open world economies. Here we have developed a SARS-CoV-2 antigen test in an inexpensive lateral flow format to generate a chromatographic result identifying the presence of the SARS-CoV-2 antigen, and thus an active infection, within a patient anterior nares swab sample. Our 15-min test requires no equipment or laboratory infrastructure to administer with a limit of detection of 2.0 × 10[SUP]2[/SUP] TCID[SUB]50[/SUB]/mL and 87.5% sensitivity, 100% specificity when tested against 40 known positive and 40 known negative patient samples established by a validated RT-qPCR test.