tetano
Editor, Senior Moderator
Sci Rep
. 2022 Jul 1;12(1):11125.
doi: 10.1038/s41598-022-15258-8.
A novel hamster model of SARS-CoV-2 respiratory infection using a pseudotyped virus
Hiroshi Yamada[SUP] 1 [/SUP], So-Ichiro Sasaki[SUP] 2 [/SUP], Hideki Tani[SUP] 3 [/SUP], Mayu Somekawa[SUP] 1 [/SUP], Hitoshi Kawasuji[SUP] 4 [/SUP], Yumiko Saga[SUP] 3 [/SUP], Yoshihiro Yoshida[SUP] 1 [/SUP], Yoshihiro Yamamoto[SUP] 4 [/SUP], Yoshihiro Hayakawa[SUP] 2 [/SUP], Yoshitomo Morinaga[SUP] 5 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a biosafety level (BSL)-3 pathogen; therefore, its research environment is limited. Pseudotyped viruses that mimic the infection of SARS-CoV-2 have been widely used for in vitro evaluation because they are available in BSL-2 containment laboratories. However, in vivo application is inadequate. Therefore, animal models instigated with animal BSL-2 will provide opportunities for in vivo evaluation. Hamsters (6-10-week-old males) were intratracheally inoculated with luciferase-expressing vesicular stomatitis virus (VSV)-based SARS-CoV-2 pseudotyped virus. The lungs were harvested 24-72 h after inoculation and luminescence was measured using an in vivo imaging system. Lung luminescence after inoculation with the SARS-CoV-2 pseudotyped virus increased in a dose-dependent manner and peaked at 48 h. The VSV-G (envelope G) pseudotyped virus also induced luminescence; however, a 100-fold concentration was required to reach a level similar to that of the SARS-CoV-2 pseudotyped virus. The SARS-CoV-2 pseudotyped virus is applicable to SARS-CoV-2 respiratory infections in a hamster model. Because of the single-round infectious virus, the model can be used to study the steps from viral binding to entry, which will be useful for future research on SARS-CoV-2 entry without using live SARS-CoV-2 or transgenic animals.
. 2022 Jul 1;12(1):11125.
doi: 10.1038/s41598-022-15258-8.
A novel hamster model of SARS-CoV-2 respiratory infection using a pseudotyped virus
Hiroshi Yamada[SUP] 1 [/SUP], So-Ichiro Sasaki[SUP] 2 [/SUP], Hideki Tani[SUP] 3 [/SUP], Mayu Somekawa[SUP] 1 [/SUP], Hitoshi Kawasuji[SUP] 4 [/SUP], Yumiko Saga[SUP] 3 [/SUP], Yoshihiro Yoshida[SUP] 1 [/SUP], Yoshihiro Yamamoto[SUP] 4 [/SUP], Yoshihiro Hayakawa[SUP] 2 [/SUP], Yoshitomo Morinaga[SUP] 5 [/SUP]
Affiliations
- PMID: 35778450
- DOI: 10.1038/s41598-022-15258-8
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a biosafety level (BSL)-3 pathogen; therefore, its research environment is limited. Pseudotyped viruses that mimic the infection of SARS-CoV-2 have been widely used for in vitro evaluation because they are available in BSL-2 containment laboratories. However, in vivo application is inadequate. Therefore, animal models instigated with animal BSL-2 will provide opportunities for in vivo evaluation. Hamsters (6-10-week-old males) were intratracheally inoculated with luciferase-expressing vesicular stomatitis virus (VSV)-based SARS-CoV-2 pseudotyped virus. The lungs were harvested 24-72 h after inoculation and luminescence was measured using an in vivo imaging system. Lung luminescence after inoculation with the SARS-CoV-2 pseudotyped virus increased in a dose-dependent manner and peaked at 48 h. The VSV-G (envelope G) pseudotyped virus also induced luminescence; however, a 100-fold concentration was required to reach a level similar to that of the SARS-CoV-2 pseudotyped virus. The SARS-CoV-2 pseudotyped virus is applicable to SARS-CoV-2 respiratory infections in a hamster model. Because of the single-round infectious virus, the model can be used to study the steps from viral binding to entry, which will be useful for future research on SARS-CoV-2 entry without using live SARS-CoV-2 or transgenic animals.