tetano
Editor, Senior Moderator
Sci Rep
. 2024 Sep 20;14(1):21961.
doi: 10.1038/s41598-024-72417-9. A colorimetric reverse-transcription loop-mediated isothermal amplification method targeting the L452R mutation to detect the Delta variant of SARS-CoV-2
Narin Thippornchai[SUP] 1 [/SUP], Sukanya Pengpanich[SUP] 2 [/SUP], Wansadaj Jaroenram[SUP] 2 [/SUP], Nathamon Kosoltanapiwat[SUP] 1 [/SUP], Passanesh Sukphopetch[SUP] 1 [/SUP], Wansika Kiatpathomchai[SUP] 3 [/SUP], Pornsawan Leaungwutiwong[SUP] 4 [/SUP]
Affiliations
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered global difficulties for both individuals and economies, with new variants continuing to emerge. The Delta variant of SARS-CoV-2 remains most prevalent worldwide, and it affects the efficacy of coronavirus disease 2019 (COVID-19) vaccination. Expedited testing to detect the Delta variant of SARS-CoV-2 and monitor viral transmission is necessary. This study aimed to develop and evaluate a colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) technique targeting the L452R mutation in the S gene for the specific detection of the Delta variant. In the test, positivity was indicated as a color change from purple to yellow. The assay's 95% limit of detection was 57 copies per reaction for the L452R (U1355G)-specific standard plasmid. Using 126 clinical samples, our assay displayed 100% specificity, 97.06% sensitivity, and 98.41% accuracy in identifying the Delta variant of SARS-CoV-2 compared to real-time RT-PCR. To our knowledge, this is the first colorimetric RT-LAMP assay that can differentiate the Delta variant from its generic SARS-CoV-2, enabling it as an approach for studying COVID-19 demography and facilitating proper effective control measure establishment to fight against the reemerging variants of SARS-CoV-2 in the future.
Keywords: Colorimetric RT-LAMP; Delta variant; Diagnostic test; L452R mutation; SARS-CoV-2.
. 2024 Sep 20;14(1):21961.
doi: 10.1038/s41598-024-72417-9. A colorimetric reverse-transcription loop-mediated isothermal amplification method targeting the L452R mutation to detect the Delta variant of SARS-CoV-2
Narin Thippornchai[SUP] 1 [/SUP], Sukanya Pengpanich[SUP] 2 [/SUP], Wansadaj Jaroenram[SUP] 2 [/SUP], Nathamon Kosoltanapiwat[SUP] 1 [/SUP], Passanesh Sukphopetch[SUP] 1 [/SUP], Wansika Kiatpathomchai[SUP] 3 [/SUP], Pornsawan Leaungwutiwong[SUP] 4 [/SUP]
Affiliations
- PMID: 39304686
- PMCID: PMC11415516
- DOI: 10.1038/s41598-024-72417-9
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered global difficulties for both individuals and economies, with new variants continuing to emerge. The Delta variant of SARS-CoV-2 remains most prevalent worldwide, and it affects the efficacy of coronavirus disease 2019 (COVID-19) vaccination. Expedited testing to detect the Delta variant of SARS-CoV-2 and monitor viral transmission is necessary. This study aimed to develop and evaluate a colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) technique targeting the L452R mutation in the S gene for the specific detection of the Delta variant. In the test, positivity was indicated as a color change from purple to yellow. The assay's 95% limit of detection was 57 copies per reaction for the L452R (U1355G)-specific standard plasmid. Using 126 clinical samples, our assay displayed 100% specificity, 97.06% sensitivity, and 98.41% accuracy in identifying the Delta variant of SARS-CoV-2 compared to real-time RT-PCR. To our knowledge, this is the first colorimetric RT-LAMP assay that can differentiate the Delta variant from its generic SARS-CoV-2, enabling it as an approach for studying COVID-19 demography and facilitating proper effective control measure establishment to fight against the reemerging variants of SARS-CoV-2 in the future.
Keywords: Colorimetric RT-LAMP; Delta variant; Diagnostic test; L452R mutation; SARS-CoV-2.