tetano
Editor, Senior Moderator
Sci Immunol
. 2020 Jul 10;5(49):eabd1554.
doi: 10.1126/sciimmunol.abd1554.
Immunophenotyping of COVID-19 and Influenza Highlights the Role of Type I Interferons in Development of Severe COVID-19
Jeong Seok Lee[SUP] #[/SUP][SUP] 1 [/SUP], Seongwan Park[SUP] #[/SUP][SUP] 2 [/SUP], Hye Won Jeong[SUP] #[/SUP][SUP] 3 [/SUP], Jin Young Ahn[SUP] #[/SUP][SUP] 4 [/SUP], Seong Jin Choi[SUP] 1 [/SUP], Hoyoung Lee[SUP] 1 [/SUP], Baekgyu Choi[SUP] 2 [/SUP], Su Kyung Nam[SUP] 2 [/SUP], Moa Sa[SUP] 1 5 [/SUP], Ji-Soo Kwon[SUP] 1 6 [/SUP], Su Jin Jeong[SUP] 4 [/SUP], Heung Kyu Lee[SUP] 1 5 [/SUP], Sung Ho Park[SUP] 7 [/SUP], Su-Hyung Park[SUP] 1 5 [/SUP], Jun Yong Choi[SUP] 8 [/SUP], Sung-Han Kim[SUP] 9 [/SUP], Inkyung Jung[SUP] 10 [/SUP], Eui-Cheol Shin[SUP] 11 5 [/SUP]
Affiliations
Abstract
Although most SARS-CoV-2-infected individuals experience mild coronavirus disease 2019 (COVID-19), some patients suffer from severe COVID-19, which is accompanied by acute respiratory distress syndrome and systemic inflammation. To identify factors driving severe progression of COVID-19, we performed single-cell RNA-seq using peripheral blood mononuclear cells (PBMCs) obtained from healthy donors, patients with mild or severe COVID-19, and patients with severe influenza. Patients with COVID-19 exhibited hyper-inflammatory signatures across all types of cells among PBMCs, particularly up-regulation of the TNF/IL-1β-driven inflammatory response as compared to severe influenza. In classical monocytes from patients with severe COVID-19, type I IFN response co-existed with the TNF/IL-1β-driven inflammation, and this was not seen in patients with milder COVID-19. Interestingly, we documented type I IFN-driven inflammatory features in patients with severe influenza as well. Based on this, we propose that the type I IFN response plays a pivotal role in exacerbating inflammation in severe COVID-19.
. 2020 Jul 10;5(49):eabd1554.
doi: 10.1126/sciimmunol.abd1554.
Immunophenotyping of COVID-19 and Influenza Highlights the Role of Type I Interferons in Development of Severe COVID-19
Jeong Seok Lee[SUP] #[/SUP][SUP] 1 [/SUP], Seongwan Park[SUP] #[/SUP][SUP] 2 [/SUP], Hye Won Jeong[SUP] #[/SUP][SUP] 3 [/SUP], Jin Young Ahn[SUP] #[/SUP][SUP] 4 [/SUP], Seong Jin Choi[SUP] 1 [/SUP], Hoyoung Lee[SUP] 1 [/SUP], Baekgyu Choi[SUP] 2 [/SUP], Su Kyung Nam[SUP] 2 [/SUP], Moa Sa[SUP] 1 5 [/SUP], Ji-Soo Kwon[SUP] 1 6 [/SUP], Su Jin Jeong[SUP] 4 [/SUP], Heung Kyu Lee[SUP] 1 5 [/SUP], Sung Ho Park[SUP] 7 [/SUP], Su-Hyung Park[SUP] 1 5 [/SUP], Jun Yong Choi[SUP] 8 [/SUP], Sung-Han Kim[SUP] 9 [/SUP], Inkyung Jung[SUP] 10 [/SUP], Eui-Cheol Shin[SUP] 11 5 [/SUP]
Affiliations
- PMID: 32651212
- DOI: 10.1126/sciimmunol.abd1554
Abstract
Although most SARS-CoV-2-infected individuals experience mild coronavirus disease 2019 (COVID-19), some patients suffer from severe COVID-19, which is accompanied by acute respiratory distress syndrome and systemic inflammation. To identify factors driving severe progression of COVID-19, we performed single-cell RNA-seq using peripheral blood mononuclear cells (PBMCs) obtained from healthy donors, patients with mild or severe COVID-19, and patients with severe influenza. Patients with COVID-19 exhibited hyper-inflammatory signatures across all types of cells among PBMCs, particularly up-regulation of the TNF/IL-1β-driven inflammatory response as compared to severe influenza. In classical monocytes from patients with severe COVID-19, type I IFN response co-existed with the TNF/IL-1β-driven inflammation, and this was not seen in patients with milder COVID-19. Interestingly, we documented type I IFN-driven inflammatory features in patients with severe influenza as well. Based on this, we propose that the type I IFN response plays a pivotal role in exacerbating inflammation in severe COVID-19.