tetano
Editor, Senior Moderator
Sci Immunol
. 2025 Jan 24;10(103):eado9572.
doi: 10.1126/sciimmunol.ado9572. Epub 2025 Jan 24. Human naïve B cells recognize prepandemic influenza virus hemagglutinins
Jared Feldman[SUP] 1 [/SUP], Ana Sofia Ferreira Ramos[SUP] 2 [/SUP], Mya Vu[SUP] 1 [/SUP], Daniel P Maurer[SUP] 1 [/SUP], Victoria C Rosado[SUP] 1 [/SUP], Daniel Lingwood[SUP] 1 [/SUP], Goran Bajic[SUP] 2 [/SUP], Aaron G Schmidt[SUP] 1 3 [/SUP]
Affiliations
Understanding the naïve B cell repertoire and its specificity for potential zoonotic threats, such as the highly pathogenic avian influenza (HPAI) H5Nx viruses, may allow prediction of infection- or vaccine-specific responses. However, this naïve repertoire and the possibility to respond to emerging, prepandemic viruses are largely undetermined. Here, we profiled naïve B cell reactivity against a prototypical HPAI H5 hemagglutinin (HA), the major target of antibody responses. We found that the frequency of H5-specific human naïve B cells targeting the HA "head" domain was increased relative to cross-reactive B cells to a circulating seasonal H1N1 strain. We classified the isolated monoclonal antibodies (mAbs) by the HA epitopes engaged and found that selected mAbs neutralized H5N1 at germline. We determined a cryo-electron microscopic structure of one mAb in complex with H5 HA to define its epitope. Our study defines the naïve human B cell repertoire recognizing a potentially zoonotic HPAI.
. 2025 Jan 24;10(103):eado9572.
doi: 10.1126/sciimmunol.ado9572. Epub 2025 Jan 24. Human naïve B cells recognize prepandemic influenza virus hemagglutinins
Jared Feldman[SUP] 1 [/SUP], Ana Sofia Ferreira Ramos[SUP] 2 [/SUP], Mya Vu[SUP] 1 [/SUP], Daniel P Maurer[SUP] 1 [/SUP], Victoria C Rosado[SUP] 1 [/SUP], Daniel Lingwood[SUP] 1 [/SUP], Goran Bajic[SUP] 2 [/SUP], Aaron G Schmidt[SUP] 1 3 [/SUP]
Affiliations
- PMID: 39854479
- DOI: 10.1126/sciimmunol.ado9572
Understanding the naïve B cell repertoire and its specificity for potential zoonotic threats, such as the highly pathogenic avian influenza (HPAI) H5Nx viruses, may allow prediction of infection- or vaccine-specific responses. However, this naïve repertoire and the possibility to respond to emerging, prepandemic viruses are largely undetermined. Here, we profiled naïve B cell reactivity against a prototypical HPAI H5 hemagglutinin (HA), the major target of antibody responses. We found that the frequency of H5-specific human naïve B cells targeting the HA "head" domain was increased relative to cross-reactive B cells to a circulating seasonal H1N1 strain. We classified the isolated monoclonal antibodies (mAbs) by the HA epitopes engaged and found that selected mAbs neutralized H5N1 at germline. We determined a cryo-electron microscopic structure of one mAb in complex with H5 HA to define its epitope. Our study defines the naïve human B cell repertoire recognizing a potentially zoonotic HPAI.