tetano
Editor, Senior Moderator
Sci Immunol
. 2021 Jul 1;6(61):eabg5669.
doi: 10.1126/sciimmunol.abg5669.
CD8 [SUP]+[/SUP] T cells specific for conserved coronavirus epitopes correlate with milder disease in COVID-19 patients
Vamsee Mallajosyula[SUP] 1 [/SUP], Conner Ganjavi[SUP] 2 [/SUP], Saborni Chakraborty[SUP] 3 [/SUP], Alana M McSween[SUP] 1 [/SUP], Ana Jimena Pavlovitch-Bedzyk[SUP] 4 [/SUP], Julie Wilhelmy[SUP] 1 [/SUP], Allison Nau[SUP] 1 [/SUP], Monali Manohar[SUP] 5 [/SUP], Kari C Nadeau[SUP] 1 5 [/SUP], Mark M Davis[SUP] 6 7 8 [/SUP]
Affiliations
Abstract
A central feature of the SARS-CoV-2 pandemic is that some individuals become severely ill or die, whereas others have only a mild disease course or are asymptomatic. Here we report development of an improved multimeric αβ T cell staining reagent platform, with each maxi-ferritin "spheromer" displaying 12 peptide-MHC complexes. Spheromers stain specific T cells more efficiently than peptide-MHC tetramers and capture a broader portion of the sequence repertoire for a given peptide-MHC. Analyzing the response in unexposed individuals, we find that T cells recognizing peptides conserved amongst coronaviruses are more abundant and tend to have a "memory" phenotype, compared to those unique to SARS-CoV-2. Significantly, CD8[SUP]+[/SUP] T cells with these conserved specificities are much more abundant in COVID-19 patients with mild disease versus those with a more severe illness, suggesting a protective role.
. 2021 Jul 1;6(61):eabg5669.
doi: 10.1126/sciimmunol.abg5669.
CD8 [SUP]+[/SUP] T cells specific for conserved coronavirus epitopes correlate with milder disease in COVID-19 patients
Vamsee Mallajosyula[SUP] 1 [/SUP], Conner Ganjavi[SUP] 2 [/SUP], Saborni Chakraborty[SUP] 3 [/SUP], Alana M McSween[SUP] 1 [/SUP], Ana Jimena Pavlovitch-Bedzyk[SUP] 4 [/SUP], Julie Wilhelmy[SUP] 1 [/SUP], Allison Nau[SUP] 1 [/SUP], Monali Manohar[SUP] 5 [/SUP], Kari C Nadeau[SUP] 1 5 [/SUP], Mark M Davis[SUP] 6 7 8 [/SUP]
Affiliations
- PMID: 34210785
- DOI: 10.1126/sciimmunol.abg5669
Abstract
A central feature of the SARS-CoV-2 pandemic is that some individuals become severely ill or die, whereas others have only a mild disease course or are asymptomatic. Here we report development of an improved multimeric αβ T cell staining reagent platform, with each maxi-ferritin "spheromer" displaying 12 peptide-MHC complexes. Spheromers stain specific T cells more efficiently than peptide-MHC tetramers and capture a broader portion of the sequence repertoire for a given peptide-MHC. Analyzing the response in unexposed individuals, we find that T cells recognizing peptides conserved amongst coronaviruses are more abundant and tend to have a "memory" phenotype, compared to those unique to SARS-CoV-2. Significantly, CD8[SUP]+[/SUP] T cells with these conserved specificities are much more abundant in COVID-19 patients with mild disease versus those with a more severe illness, suggesting a protective role.