tetano
Editor, Senior Moderator
Sci China Life Sci
. 2024 Mar 8.
doi: 10.1007/s11427-023-2484-x. Online ahead of print. Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor
Luo-Yuan Xia[SUP] #[/SUP][SUP] 1 2 [/SUP], Xue-Feng Wang[SUP] #[/SUP][SUP] 3 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 2 4 [/SUP], Yi-Ming Zhang[SUP] #[/SUP][SUP] 3 5 [/SUP], Zhen-Fei Wang[SUP] #[/SUP][SUP] 1 [/SUP], En-Tao Li[SUP] #[/SUP][SUP] 3 [/SUP], Chang-Fa Fan[SUP] 6 [/SUP], Ke Song[SUP] 1 [/SUP], Yuan-Guo Li[SUP] 3 [/SUP], Run-Ze Ye[SUP] 1 2 [/SUP], Fang-Xu Li[SUP] 3 [/SUP], Dai-Yun Zhu[SUP] 2 [/SUP], Jie Zhang[SUP] 2 [/SUP], Zhuang-Zhuang Shi[SUP] 3 [/SUP], Ming-Zhu Zhang[SUP] 2 [/SUP], Liang-Jing Li[SUP] 2 [/SUP], Shi-Jing Shen[SUP] 2 [/SUP], Song Jin[SUP] 3 [/SUP], Ya-Wei Zhang[SUP] 2 [/SUP], Wei-Guang Fu[SUP] 3 [/SUP], Lin Zhao[SUP] 1 [/SUP], Wen-Hao Wang[SUP] 3 [/SUP], Tie-Cheng Wang[SUP] 3 [/SUP], You-Chun Wang[SUP] 7 [/SUP], Jia-Fu Jiang[SUP] 2 4 [/SUP], Yan-Ling Hu[SUP] 8 [/SUP], Na Jia[SUP] 9 10 [/SUP], Yu-Wei Gao[SUP] 11 [/SUP], Wu-Chun Cao[SUP] 12 13 14 [/SUP]
Affiliations
Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins, showing a potential threat to humans. Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus, PCoV-GX/P2V, which was isolated from a Malayan pangolin (Manis javanica). PCoV-GX/P2V could grow in human hepatoma, colorectal adenocarcinoma cells, and human primary nasal epithelial cells. It replicated more efficiently in cells expressing human angiotensin-converting enzyme 2 (hACE2) as SARS-CoV-2 did. After intranasal inoculation to the hACE2-transgenic mice, PCoV-GX/P2V not only replicated in nasal turbinate and lungs, but also caused interstitial pneumonia, characterized by infiltration of mixed inflammatory cells and multifocal alveolar hemorrhage. Existing population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V infection. These findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission.
Keywords: SARS-CoV-2-related virus; hACE2-transgenic mice; human ACE2 receptor; human cell lines; infectivity; pangolin; pathogenicity.
. 2024 Mar 8.
doi: 10.1007/s11427-023-2484-x. Online ahead of print. Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor
Luo-Yuan Xia[SUP] #[/SUP][SUP] 1 2 [/SUP], Xue-Feng Wang[SUP] #[/SUP][SUP] 3 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 2 4 [/SUP], Yi-Ming Zhang[SUP] #[/SUP][SUP] 3 5 [/SUP], Zhen-Fei Wang[SUP] #[/SUP][SUP] 1 [/SUP], En-Tao Li[SUP] #[/SUP][SUP] 3 [/SUP], Chang-Fa Fan[SUP] 6 [/SUP], Ke Song[SUP] 1 [/SUP], Yuan-Guo Li[SUP] 3 [/SUP], Run-Ze Ye[SUP] 1 2 [/SUP], Fang-Xu Li[SUP] 3 [/SUP], Dai-Yun Zhu[SUP] 2 [/SUP], Jie Zhang[SUP] 2 [/SUP], Zhuang-Zhuang Shi[SUP] 3 [/SUP], Ming-Zhu Zhang[SUP] 2 [/SUP], Liang-Jing Li[SUP] 2 [/SUP], Shi-Jing Shen[SUP] 2 [/SUP], Song Jin[SUP] 3 [/SUP], Ya-Wei Zhang[SUP] 2 [/SUP], Wei-Guang Fu[SUP] 3 [/SUP], Lin Zhao[SUP] 1 [/SUP], Wen-Hao Wang[SUP] 3 [/SUP], Tie-Cheng Wang[SUP] 3 [/SUP], You-Chun Wang[SUP] 7 [/SUP], Jia-Fu Jiang[SUP] 2 4 [/SUP], Yan-Ling Hu[SUP] 8 [/SUP], Na Jia[SUP] 9 10 [/SUP], Yu-Wei Gao[SUP] 11 [/SUP], Wu-Chun Cao[SUP] 12 13 14 [/SUP]
Affiliations
- PMID: 38478297
- DOI: 10.1007/s11427-023-2484-x
Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins, showing a potential threat to humans. Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus, PCoV-GX/P2V, which was isolated from a Malayan pangolin (Manis javanica). PCoV-GX/P2V could grow in human hepatoma, colorectal adenocarcinoma cells, and human primary nasal epithelial cells. It replicated more efficiently in cells expressing human angiotensin-converting enzyme 2 (hACE2) as SARS-CoV-2 did. After intranasal inoculation to the hACE2-transgenic mice, PCoV-GX/P2V not only replicated in nasal turbinate and lungs, but also caused interstitial pneumonia, characterized by infiltration of mixed inflammatory cells and multifocal alveolar hemorrhage. Existing population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V infection. These findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission.
Keywords: SARS-CoV-2-related virus; hACE2-transgenic mice; human ACE2 receptor; human cell lines; infectivity; pangolin; pathogenicity.