tetano
Editor, Senior Moderator
Sci Adv
. 2025 Jun 13;11(24):eadu7602.
doi: 10.1126/sciadv.adu7602. Epub 2025 Jun 11. Influenza A virus subverts the LC3-pericentrin dynein adaptor complex for host cytoplasm entry
Yingying Cong[SUP] 1 2 3 [/SUP], Pauline Verlhac[SUP] 3 [/SUP], Benjamin B Green[SUP] 4 [/SUP], Jacqueline de Vries-Idema[SUP] 5 [/SUP], Line Moesgaard Strauss[SUP] 1 [/SUP], Clàudia Río-Bergé[SUP] 1 [/SUP], Anders Etzerodt[SUP] 1 [/SUP], Lene N Nejsum[SUP] 4 [/SUP], Anke L W Huckriede[SUP] 5 [/SUP], Fulvio Reggiori[SUP] 1 3 6 [/SUP]
Affiliations
Influenza A virus (IAV) enters host cells via endocytosis, and fusion of the viral particles (VPs) at endosomes releases the viral ribonucleoproteins (vRNPs) into the cytoplasm. This uncoating step that is vital for IAV infection remains to be fully understood. The aggresome processing machinery (APM) plays a relevant but not essential role in this. Here, we reveal a mechanism in which light chain 3 proteins (LC3s) and pericentrin (PCNT) form an adaptor complex that is required for vRNPs binding to the dynein 1 and IAV uncoating at endosomes. This function of LC3s and PCNT is independent from their established role in autophagy and centrosome assembly, respectively. LC3s or PCNT depletion severely impairs IAV cytoplasm entry and infection, which can be further inhibited by additional silencing of histone deacetylase 6, an APM component. Collectively, our results show that IAV has adopted two redundant strategies to hijack the dynein biomolecular motors and facilitate VP uncoating.
. 2025 Jun 13;11(24):eadu7602.
doi: 10.1126/sciadv.adu7602. Epub 2025 Jun 11. Influenza A virus subverts the LC3-pericentrin dynein adaptor complex for host cytoplasm entry
Yingying Cong[SUP] 1 2 3 [/SUP], Pauline Verlhac[SUP] 3 [/SUP], Benjamin B Green[SUP] 4 [/SUP], Jacqueline de Vries-Idema[SUP] 5 [/SUP], Line Moesgaard Strauss[SUP] 1 [/SUP], Clàudia Río-Bergé[SUP] 1 [/SUP], Anders Etzerodt[SUP] 1 [/SUP], Lene N Nejsum[SUP] 4 [/SUP], Anke L W Huckriede[SUP] 5 [/SUP], Fulvio Reggiori[SUP] 1 3 6 [/SUP]
Affiliations
- PMID: 40498831
- PMCID: PMC12154174
- DOI: 10.1126/sciadv.adu7602
Influenza A virus (IAV) enters host cells via endocytosis, and fusion of the viral particles (VPs) at endosomes releases the viral ribonucleoproteins (vRNPs) into the cytoplasm. This uncoating step that is vital for IAV infection remains to be fully understood. The aggresome processing machinery (APM) plays a relevant but not essential role in this. Here, we reveal a mechanism in which light chain 3 proteins (LC3s) and pericentrin (PCNT) form an adaptor complex that is required for vRNPs binding to the dynein 1 and IAV uncoating at endosomes. This function of LC3s and PCNT is independent from their established role in autophagy and centrosome assembly, respectively. LC3s or PCNT depletion severely impairs IAV cytoplasm entry and infection, which can be further inhibited by additional silencing of histone deacetylase 6, an APM component. Collectively, our results show that IAV has adopted two redundant strategies to hijack the dynein biomolecular motors and facilitate VP uncoating.