tetano
Editor, Senior Moderator
Sci Adv
. 2020 Jun 17;6(25):eabb5813.
doi: 10.1126/sciadv.abb5813. eCollection 2020 Jun.
Evidence for Host-Dependent RNA Editing in the Transcriptome of SARS-CoV-2
Salvatore Di Giorgio[SUP] 1 2 [/SUP], Filippo Martignano[SUP] 1 2 [/SUP], Maria Gabriella Torcia[SUP] 3 [/SUP], Giorgio Mattiuz[SUP] 1 3 [/SUP], Silvestro G Conticello[SUP] 1 4 [/SUP]
AffiliationsExpand
Abstract
The COVID-19 outbreak has become a global health risk, and understanding the response of the host to the SARS-CoV-2 virus will help to combat the disease. RNA editing by host deaminases is an innate restriction process to counter virus infection, but it is not yet known whether this process operates against coronaviruses. Here, we analyze RNA sequences from bronchoalveolar lavage fluids obtained from coronavirus-infected patients. We identify nucleotide changes that may be signatures of RNA editing: adenosine-to-inosine changes from ADAR deaminases and cytosine-to-uracil changes from APOBEC deaminases. Mutational analysis of genomes from different strains of Coronaviridae from human hosts reveals mutational patterns consistent with those observed in the transcriptomic data. However, the reduced ADAR signature in these data raises the possibility that ADARs might be more effective than APOBECs in restricting viral propagation. Our results thus suggest that both APOBECs and ADARs are involved in coronavirus genome editing, a process that may shape the fate of both virus and patient.
. 2020 Jun 17;6(25):eabb5813.
doi: 10.1126/sciadv.abb5813. eCollection 2020 Jun.
Evidence for Host-Dependent RNA Editing in the Transcriptome of SARS-CoV-2
Salvatore Di Giorgio[SUP] 1 2 [/SUP], Filippo Martignano[SUP] 1 2 [/SUP], Maria Gabriella Torcia[SUP] 3 [/SUP], Giorgio Mattiuz[SUP] 1 3 [/SUP], Silvestro G Conticello[SUP] 1 4 [/SUP]
AffiliationsExpand
- PMID: 32596474
- PMCID: PMC7299625
- DOI: 10.1126/sciadv.abb5813
Abstract
The COVID-19 outbreak has become a global health risk, and understanding the response of the host to the SARS-CoV-2 virus will help to combat the disease. RNA editing by host deaminases is an innate restriction process to counter virus infection, but it is not yet known whether this process operates against coronaviruses. Here, we analyze RNA sequences from bronchoalveolar lavage fluids obtained from coronavirus-infected patients. We identify nucleotide changes that may be signatures of RNA editing: adenosine-to-inosine changes from ADAR deaminases and cytosine-to-uracil changes from APOBEC deaminases. Mutational analysis of genomes from different strains of Coronaviridae from human hosts reveals mutational patterns consistent with those observed in the transcriptomic data. However, the reduced ADAR signature in these data raises the possibility that ADARs might be more effective than APOBECs in restricting viral propagation. Our results thus suggest that both APOBECs and ADARs are involved in coronavirus genome editing, a process that may shape the fate of both virus and patient.