tetano
Editor, Senior Moderator
Pediatr Res. 2012 Nov 30. doi: 10.1038/pr.2012.176. [Epub ahead of print]
RT-PCR detection of respiratory pathogens in newborn children admitted to a neonatal medium care unit.
Smit PM, Pronk SM, Kaandorp JC, Weijer O, Lauw FN, Smits PH, Claas EC, Mulder JW, Beijnen JH, Brandjes DP.
Source
Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands.
Abstract
Background:This study aimed to determine the prevalence of respiratory pathogens among newborns admitted to a neonatal medium care unit (NMCU) and to identify clinical predictors.Methods:A 1-year observational study was performed of neonates admitted to an NMCU in Amsterdam, The Netherlands. Nasopharyngeal samples were collected for detection of respiratory viruses and bacteria by RT-PCR. Cycle threshold (Ct) values were provided to estimate viral load. Predictors for the presence of study pathogens were identified.Results:From October 2010 through September 2011, 334 neonates (median age 1.3 days, 53.6% male) were included. Overall, 37 respiratory pathogens were detected in 34 children (10.2%): parainfluenza-1 (n=9), human rhinovirus (n=7), parainfluenza-3 (n=6), respiratory syncytial virus (n=6), Streptococcus pneumoniae (n=3), adenovirus (n=2), human coronavirus (n=2), influenza A (n=1) and bocavirus (n=1). Neonates with higher viral loads (Ct <35; n=11) were more often clinically ill than those with lower viral loads (Ct ≥35; n=23). Two variables significantly contributed to the detection of study pathogens: age (OR 1.21 for each day older; 95% CI 1.12-1.30) and rhinorrhea (OR 6.71; 95% CI 1.54-29.21).Conclusion:Respiratory pathogens seem to play a role in neonates admitted to an NMCU. Whether this will influence clinical management remains to be answered.Pediatric Research (2012); doi:10.1038/pr.2012.176.
PMID:
23202720
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23202720
RT-PCR detection of respiratory pathogens in newborn children admitted to a neonatal medium care unit.
Smit PM, Pronk SM, Kaandorp JC, Weijer O, Lauw FN, Smits PH, Claas EC, Mulder JW, Beijnen JH, Brandjes DP.
Source
Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands.
Abstract
Background:This study aimed to determine the prevalence of respiratory pathogens among newborns admitted to a neonatal medium care unit (NMCU) and to identify clinical predictors.Methods:A 1-year observational study was performed of neonates admitted to an NMCU in Amsterdam, The Netherlands. Nasopharyngeal samples were collected for detection of respiratory viruses and bacteria by RT-PCR. Cycle threshold (Ct) values were provided to estimate viral load. Predictors for the presence of study pathogens were identified.Results:From October 2010 through September 2011, 334 neonates (median age 1.3 days, 53.6% male) were included. Overall, 37 respiratory pathogens were detected in 34 children (10.2%): parainfluenza-1 (n=9), human rhinovirus (n=7), parainfluenza-3 (n=6), respiratory syncytial virus (n=6), Streptococcus pneumoniae (n=3), adenovirus (n=2), human coronavirus (n=2), influenza A (n=1) and bocavirus (n=1). Neonates with higher viral loads (Ct <35; n=11) were more often clinically ill than those with lower viral loads (Ct ≥35; n=23). Two variables significantly contributed to the detection of study pathogens: age (OR 1.21 for each day older; 95% CI 1.12-1.30) and rhinorrhea (OR 6.71; 95% CI 1.54-29.21).Conclusion:Respiratory pathogens seem to play a role in neonates admitted to an NMCU. Whether this will influence clinical management remains to be answered.Pediatric Research (2012); doi:10.1038/pr.2012.176.
PMID:
23202720
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23202720