tetano
Editor, Senior Moderator
Transbound Emerg Dis. 2019 Nov 12. doi: 10.1111/tbed.13411. [Epub ahead of print] [h=1]Reverse transcription recombinase-aided amplification assay for H7 subtype avian influenza virus.[/h]
Wang S[SUP]1[/SUP], Huang B[SUP]1[/SUP], Ma X[SUP]2[/SUP], Liu P[SUP]1[/SUP], Wang Y[SUP]3[/SUP], Zhang X[SUP]2[/SUP], Zhu L[SUP]1[/SUP], Fan Q[SUP]3[/SUP], Sun Y[SUP]3[/SUP], Wang K[SUP]1[/SUP].
[h=3]Author information[/h] 1 China Animal Health and Epidemiology Center, Qingdao, Shandong Province, China. 2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. 3 College of Animal Science and Technology, Henan University of Science and Technology, Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang.
[h=3]Abstract[/h] H7 subtype avian influenza virus infection is an emerging zoonosis in some Asian countries and an important avian disease worldwide. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse transcription recombinase-aided amplification assay for the detection of H7 subtype avian influenza virus. Assays were performed at a single temperature (39?C) and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus, which are the other main respiratory viruses affecting birds. The analytical sensitivity was 10[SUP]2[/SUP] RNA copies per reaction at a 95% probability level according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the reverse transcription recombinase-aided amplification assay in 342 avian clinical samples was 0.988 (p < .001). The sensitivity for avian clinical sample detection was 100% (95%CI, 90.40%-100%) and the specificity was 99.96% (95%CI, 97.83%-99.98%). These results indicated that our reverse transcription recombinase-aided amplification assay may be a valuable tool for detecting avian influenza H7 subtype virus.
? 2019 Blackwell Verlag GmbH.
[h=4]KEYWORDS:[/h] H7 subtype; avian influenza; reverse transcription recombinase-aided amplification assay
PMID: 31714018 DOI: 10.1111/tbed.13411
Wang S[SUP]1[/SUP], Huang B[SUP]1[/SUP], Ma X[SUP]2[/SUP], Liu P[SUP]1[/SUP], Wang Y[SUP]3[/SUP], Zhang X[SUP]2[/SUP], Zhu L[SUP]1[/SUP], Fan Q[SUP]3[/SUP], Sun Y[SUP]3[/SUP], Wang K[SUP]1[/SUP].
[h=3]Author information[/h] 1 China Animal Health and Epidemiology Center, Qingdao, Shandong Province, China. 2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. 3 College of Animal Science and Technology, Henan University of Science and Technology, Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang.
[h=3]Abstract[/h] H7 subtype avian influenza virus infection is an emerging zoonosis in some Asian countries and an important avian disease worldwide. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse transcription recombinase-aided amplification assay for the detection of H7 subtype avian influenza virus. Assays were performed at a single temperature (39?C) and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus, which are the other main respiratory viruses affecting birds. The analytical sensitivity was 10[SUP]2[/SUP] RNA copies per reaction at a 95% probability level according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the reverse transcription recombinase-aided amplification assay in 342 avian clinical samples was 0.988 (p < .001). The sensitivity for avian clinical sample detection was 100% (95%CI, 90.40%-100%) and the specificity was 99.96% (95%CI, 97.83%-99.98%). These results indicated that our reverse transcription recombinase-aided amplification assay may be a valuable tool for detecting avian influenza H7 subtype virus.
? 2019 Blackwell Verlag GmbH.
[h=4]KEYWORDS:[/h] H7 subtype; avian influenza; reverse transcription recombinase-aided amplification assay
PMID: 31714018 DOI: 10.1111/tbed.13411