Reverse genetics platform construction of influenza pandemic virus strain

[tetano]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Apr;27(2):85-8.
[Reverse genetics platform construction of influenza pandemic virus strain].
[Article in Chinese]
Bo H, Zhang Y, Huang WJ, Zhao X, Guo JF, Wang DY, Shu YL.
Source

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Abstract
OBJECTIVE:

Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).
METHODS:

Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.
RESULTS:

Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.
CONCLUSION:

The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.

PMID:
24044207
[PubMed - in process]

http://www.ncbi.nlm.nih.gov/pubmed/24044207

 

[gsgs]

Re: Reverse genetics platform construction of influenza pandemic virus strain

well done. How long did it take,what did it cost,
can you easily do the same with the 1918 virus ?