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Response regarding Russian mosquito/H5N1 claim

Everyone,
I have recevied a response from the university of Florida's IFAS regarding that Russian claim that mosquitoes are a vector for H5N1.

As follows:
Dear Mr. McPherson,

In reference to your questions about mosquitoes transmitting Avian Flu, I would be happy to talk to you by phone for a more detailed discussion, but for now:

The insects listed in the Power Point presentation that you sent are all flies and beetles that are common in and around poultry operations/farms. These particular flies are attracted to the feces from the poultry. They are not blood-feeding insects. It is not surprising that these insects have been found with virus, they basically roll around in all the chicken poop ? and as explained in the article you sent, the virus is shed in the chickens feces. They may mechanically transmit the virus by having it on their foot pads, outside mouth parts, etc., but the most common method of transmission of any pathogen by mosquitoes is biological. For mosquitoes to play a significant role in transmission of a virus, biological transmission is necessary. This involves an increase in the virus inside the mosquito, after picking it up from a blood meal, and then transmission occurs sometime later when the mosquito feeds on another host. This occurs with West Nile, SLE, EEE, etc. The paper and the power point presentation include insects that are mechanical transmitters attracted to the poop. Mosquitoes are attracted to CO2, not poop, to feed on blood.

Concerning the Russian article that states that they have proven that mosquitoes transmit Avian Flu: A headline and one paragraph stating that Russians found that mosquitoes transmit Avian Flu, with absolutely no data to support this claim, does not constitute peer review. Such a finding is huge ? and "huge" gets published in Science or Nature, regardless of the language, and is/should be widely circulated. Pro-Med would have been all over it too, if it were peer reviewed and published in a credible scientific journal. An internet posting such as this one is not peer reviewed. Such reports are often made and make it to the media, but I urge caution to you and your committee in accepting this at face value.

The problem with an unsubstantiated report is that all the details are missing ? this is not good or acceptable science. There has never been any epidemiological evidence or reason to suspect that mosquitoes or bats have ever played a substantial role in a flu epidemic. If substantiated, it would just be another method of transmission and would have to be assessed in the face of aerosol, animal to animal, and animal to person mechanisms which are proven and clearly important.

While the internet has become a great means of communicating news rapidly, it does omit time for peer review and allows gossip to spread rapidly. We rely on scientific review that occurs no matter the nationality.

So, after much consideration of your questions, and discussing this among my colleagues here at UF, the bottom line is that there is no available data to substantiate this claim, including the original work, that would cause concern here, or a shift in research priorities.

Take care and please let me know if you have any questions about my statements.

Sincerely,

C. Roxanne (Rutledge) Connelly, Ph.D.
Associate Professor
Medical Entomology Extension Specialist
Univ of Florida - IFAS

Silly Russians. -- SM
 
Re: Response regarding Russian mosquito/H5N1 claim

SM, thanks for taking the time to follow up on the issue of mosquitos as H5N1 vectors. Also a large thanks to Dr. Connelly for taking the time to provide a clear, straight forward response.
 
Re: Response regarding Russian mosquito/H5N1 claim

H5N1 was in the intestines of the flies, not just mechanical
hmm, did it replicate there or just survive ? I don't remember
 
Re: Response regarding Russian mosquito/H5N1 claim

She is one of the world's experts in issues regarding mosquitos.
 
Re: Response regarding Russian mosquito/H5N1 claim

H5N1 was in the intestines of the flies, not just mechanical
hmm, did it replicate there or just survive ? I don't remember

http://www.ajtmh.org/cgi/content/abstract/75/2/327

DETECTION AND ISOLATION OF HIGHLY PATHOGENIC H5N1 AVIAN INFLUENZA A VIRUSES FROM BLOW FLIES COLLECTED IN THE VICINITY OF AN INFECTED POULTRY FARM IN KYOTO, JAPAN, 2.....

(summary...)

During the outbreak of highly pathogenic avian influenza that<SUP> </SUP>occurred in Tamba Town, Kyoto Prefecture in 2004, a total of<SUP> </SUP>926 flies were collected from six sites within a radius of 2.3<SUP> </SUP>km from the poultry farm. The H5 influenza A virus genes were<SUP> </SUP>detected from the intestinal organs, crop, and gut of the two<SUP> </SUP>blow fly species, Calliphora nigribarbis and Aldrichina grahami,<SUP> </SUP>by reverse transcription-polymerase chain reaction for the matrix<SUP> </SUP>protein (M) and hemagglutinin (HA) genes. The HA gene encoding<SUP> </SUP>multiple basic amino acids at the HA cleavage site indicated<SUP> </SUP>that this virus is a highly pathogenic strain. Based on the<SUP> </SUP>full-length sequences of the M, HA, and neuraminidase (NA) segments<SUP> </SUP>of virus isolates through embryonated chicken eggs, the virus<SUP> </SUP>from C. nigribarbis (A/blow fly/Kyoto/93/2004) was characterized<SUP> </SUP>as H5N1 subtype influenza A virus and shown to have > 99.9%<SUP> </SUP>identities in all three RNA segments to a strain from chickens<SUP> </SUP>(A/chicken/Kyoto/3/2004) and crows (A/crows/Kyoto/53/2004) derived<SUP> </SUP>during this outbreak period in Kyoto in 2004. Our results suggest<SUP> </SUP>it is possible that blow flies could become a mechanical transmitter<SUP> </SUP>of H5N1 influenza virus.<SUP> </SUP>
<SUP></SUP>
<SUP>.</SUP>
 
Re: Response regarding Russian mosquito/H5N1 claim

Concerning the Russian article that states that they have proven that mosquitoes transmit Avian Flu: A headline and one paragraph stating that Russians found that mosquitoes transmit Avian Flu, with absolutely no data to support this claim, does not constitute peer review....

The problem with an unsubstantiated report is that all the details are missing ? this is not good or acceptable science.
Well, duh -- we know all that. :rolleyes:
This involves an increase in the virus inside the mosquito, after picking it up from a blood meal....
I wonder why it would require an increase in the virus inside the mosquito? Why should that be?
Silly Russians. -- SM
Thanks for checking into this further, Scott. I recall trying to find more info on this -- and did find a little -- but it was in Russian -- and, as we all know, working from machine-translations is pretty difficult, so who knows what the whole story is here. Maybe they were just talking about mosquitoes as a mechanical vector -- I don't recall the original article(s) just now.

In any event, I wouldn't write off the Russians so quickly -- first people to put a man in space -- invented the modern helicopter -- five Nobels in physics, two in chemistry and two in medicine. Don't sell the Russians short! :)
 
Re: Response regarding Russian mosquito/H5N1 claim

This involves an increase in the virus inside the mosquito, after picking it up from a blood meal....
I wonder why it would require an increase in the virus inside the mosquito? Why should that be?
Looks like she is talking about whether the virus is able to replicate within mosquito. If it can't then it is mechanical vector.
 
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Re: Response regarding Russian mosquito/H5N1 claim

Scott said that he forwarded the entire thread to the Univ of Florida mosquito people -- but Dr. Connelly, unfortunately, doesn't seem to have read the whole thread. She only comments on the "headline and one paragraph" which, of course, doesn't tell us much.

I did, however, post a little further info directly from the Sverdlovsk Rospotrebnadzor website -- it describes how Russian virologists (claim to) have found influenza A viruses (not just H5N1) in blood in mosquitoes:
other thread said:
Machine translation:

Finally, the Russian virologists vividly illustrated by the blood of mosquitoes carrying the virus influenza group A, which applies to both traditional human strain H3N2, and H5N1 avian influenza virus.

http://www.ocsen.ru/index.php?p=741 [<< in Russian]

http://www.flutrackers.com/forum/showpost.php?p=71741&postcount=18
Unfortunately, there's no further info beyond that (like which virologists, when, etc.).

The contact at the Sverdlovsk Rospotrebnadzor is:

Entomologist of epidemiologic investigations
FGUZ - Hygiene and Epidemiology in Sverdlovsk oblasti
T. A. Pimenov
Tel. 374-12-44


Sorry, I wasn't able to find an email for Pimenov before (and I'm afraid I haven't got the time to search more tonight).
 
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Re: Response regarding Russian mosquito/H5N1 claim

bloody Russian mosquitos.

what happens with the sucked blood ? It's "converted" to eggs AFAIK.
Some few viruses could go into mosquito-saliva and then into
another mammal's blood.
Probably only good for the immune system and bad for the few viruses-
unless the load could be increased.

Also rarely are viruses in the blood (only H5N1), the victims
are very sick then, will they attract mosquitos in that shape ?

The viruses in the eggs can hardly survive ... unless they replicate.

The viruses in the mosquitos may survive some weeks or months,
but when the mosquito is full of blood, it probably withdraws from
sucking new blood and concentrates on egg-production ?
 
Re: Response regarding Russian mosquito/H5N1 claim

Everyone,
I sent the info on the Russian contact to UF/IFAS. I am sure they will follow up.
 
Re: Response regarding Russian mosquito/H5N1 claim

Wow. Great work following this up. The mosquito possibility is really scary - or at least it was. Certainly this following up ability, done in a rational and respectful manner, really sets the FT community apart.

J.
 
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