tetano
Editor, Senior Moderator
Res Sq
. 2020 May 6;rs.3.rs-27220.
doi: 10.21203/rs.3.rs-27220/v1. Preprint
B cell clonal expansion and convergent antibody responses to SARS-CoV-2
Sandra C A Nielsen[SUP] 1 [/SUP], Fan Yang[SUP] 1 [/SUP], Ramona A Hoh[SUP] 1 [/SUP], Katherine J L Jackson[SUP] 2 [/SUP], Katharina Roeltgen[SUP] 1 [/SUP], Ji-Yeun Lee[SUP] 1 [/SUP], Arjun Rustagi[SUP] 3 [/SUP], Angela J Rogers[SUP] 4 [/SUP], Abigail E Powell[SUP] 5 [/SUP], Peter S Kim[SUP] 5 [/SUP], Taia T Wang[SUP] 3 [/SUP], Benjamin Pinsky[SUP] 1 [/SUP], Catherine A Blish[SUP] 3 [/SUP], Scott D Boyd[SUP] 1 [/SUP]
Affiliations
Abstract
During virus infection B cells are critical for the production of antibodies and protective immunity. Establishment of a diverse antibody repertoire occurs by rearrangement of germline DNA at the immunoglobulin heavy and light chain loci to encode the membrane-bound form of antibodies, the B cell antigen receptor. Little is known about the B cells and antigen receptors stimulated by the novel human coronavirus SARS-CoV-2. Here we show that the human B cell compartment in patients with diagnostically confirmed SARS-CoV-2 and clinical COVID-19 is rapidly altered with the early recruitment of B cells expressing a limited subset of V genes, and extensive activation of IgG and IgA subclasses without significant somatic mutation. We detect expansion of B cell clones as well as convergent antibodies with highly similar sequences across SARS-CoV-2 patients, highlighting stereotyped na?ve responses to this virus. A shared convergent B cell clonotype in SARS-CoV-2 infected patients was previously seen in patients with SARS. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and other zoonotic spillover coronaviruses.
Keywords: B cells; SARS-CoV-2; antigen receptors.
. 2020 May 6;rs.3.rs-27220.
doi: 10.21203/rs.3.rs-27220/v1. Preprint
B cell clonal expansion and convergent antibody responses to SARS-CoV-2
Sandra C A Nielsen[SUP] 1 [/SUP], Fan Yang[SUP] 1 [/SUP], Ramona A Hoh[SUP] 1 [/SUP], Katherine J L Jackson[SUP] 2 [/SUP], Katharina Roeltgen[SUP] 1 [/SUP], Ji-Yeun Lee[SUP] 1 [/SUP], Arjun Rustagi[SUP] 3 [/SUP], Angela J Rogers[SUP] 4 [/SUP], Abigail E Powell[SUP] 5 [/SUP], Peter S Kim[SUP] 5 [/SUP], Taia T Wang[SUP] 3 [/SUP], Benjamin Pinsky[SUP] 1 [/SUP], Catherine A Blish[SUP] 3 [/SUP], Scott D Boyd[SUP] 1 [/SUP]
Affiliations
- PMID: 32702737
- PMCID: PMC7336706
- DOI: 10.21203/rs.3.rs-27220/v1
Abstract
During virus infection B cells are critical for the production of antibodies and protective immunity. Establishment of a diverse antibody repertoire occurs by rearrangement of germline DNA at the immunoglobulin heavy and light chain loci to encode the membrane-bound form of antibodies, the B cell antigen receptor. Little is known about the B cells and antigen receptors stimulated by the novel human coronavirus SARS-CoV-2. Here we show that the human B cell compartment in patients with diagnostically confirmed SARS-CoV-2 and clinical COVID-19 is rapidly altered with the early recruitment of B cells expressing a limited subset of V genes, and extensive activation of IgG and IgA subclasses without significant somatic mutation. We detect expansion of B cell clones as well as convergent antibodies with highly similar sequences across SARS-CoV-2 patients, highlighting stereotyped na?ve responses to this virus. A shared convergent B cell clonotype in SARS-CoV-2 infected patients was previously seen in patients with SARS. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and other zoonotic spillover coronaviruses.
Keywords: B cells; SARS-CoV-2; antigen receptors.