tetano
Editor, Senior Moderator
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Dec;31(12):1615-9.
[h=1][Recombinant influenza A virus NS2 protein inhibits the production of interferon in lung tissues of infected mice].[/h] [Article in Chinese]
Zhu Y[SUP]1[/SUP], Mou Q[SUP]1[/SUP], Zhao B[SUP]1[/SUP], Luo H[SUP]2[/SUP], Zhi Y[SUP]2[/SUP], Lan L[SUP]2[/SUP], Jiang Y[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective To obtain non-structural protein 2 (NS2) of influenza A virus (IAV) via the expression system of Escherichia coli, and observe the effect of NS2 on the production of interferon γ (IFN-γ) in mouse lung tissues. Methods A prokaryotic expression plasmid pET22b(+)/NS2 was constructed and transformed into Rosetta-gami(2) of Escherichia coli. Through the inducible expression and purification, NS2 was prepared. Forty BALB/c mice were divided randomly into 8 groups, normal control group, 200 μg/kg NS2 joint virus group, 200 μg/kg NS2 joint RNA group, 100 μg/kg inactivated virus group, 0.2?lethal dose 50% (LD50) virus group, viral RNA group of 50 μg/kg, 200 μg/kg NS2 group, and 100 μg/kg NS2 group. All mice were subjected to nasal inhalation. Reverse transcription-PCR and Western blotting were respectively performed to detect the mRNA and protein expressions of IFN-γ in lung tissues. Results Recombinant NS2 was obtained by the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) and purification. After the nasal inhalation of NS2 or/and IAV to mice, reverse transcription-PCR and Western blotting showed that the levels of IFN-γ mRNA and protein in the lung tissues of NS2 joint virus group were lower than those of the virus group. Conclusion NS2 could inhibit the expressions of IFN-γ mRNA and protein in lung tissues of mice infected by IAV.
PMID: 26648293 [PubMed - in process]
[h=1][Recombinant influenza A virus NS2 protein inhibits the production of interferon in lung tissues of infected mice].[/h] [Article in Chinese]
Zhu Y[SUP]1[/SUP], Mou Q[SUP]1[/SUP], Zhao B[SUP]1[/SUP], Luo H[SUP]2[/SUP], Zhi Y[SUP]2[/SUP], Lan L[SUP]2[/SUP], Jiang Y[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective To obtain non-structural protein 2 (NS2) of influenza A virus (IAV) via the expression system of Escherichia coli, and observe the effect of NS2 on the production of interferon γ (IFN-γ) in mouse lung tissues. Methods A prokaryotic expression plasmid pET22b(+)/NS2 was constructed and transformed into Rosetta-gami(2) of Escherichia coli. Through the inducible expression and purification, NS2 was prepared. Forty BALB/c mice were divided randomly into 8 groups, normal control group, 200 μg/kg NS2 joint virus group, 200 μg/kg NS2 joint RNA group, 100 μg/kg inactivated virus group, 0.2?lethal dose 50% (LD50) virus group, viral RNA group of 50 μg/kg, 200 μg/kg NS2 group, and 100 μg/kg NS2 group. All mice were subjected to nasal inhalation. Reverse transcription-PCR and Western blotting were respectively performed to detect the mRNA and protein expressions of IFN-γ in lung tissues. Results Recombinant NS2 was obtained by the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) and purification. After the nasal inhalation of NS2 or/and IAV to mice, reverse transcription-PCR and Western blotting showed that the levels of IFN-γ mRNA and protein in the lung tissues of NS2 joint virus group were lower than those of the virus group. Conclusion NS2 could inhibit the expressions of IFN-γ mRNA and protein in lung tissues of mice infected by IAV.
PMID: 26648293 [PubMed - in process]