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Source: http://7thspace.com/headlines/43412...paratransgenic_control_of_chagas_disease.html
Recombinant Arthrobacter beta-1, 3-glucanase as a potential effector molecule for paratransgenic control of Chagas disease
Chagas disease is most often transmitted to humans by Trypanosoma cruzi infected triatomine bugs, and remains a significant cause of morbidity and mortality in Central and South America. Control of Chagas disease has relied mainly on vector eradication.
However, development of insect resistance has prompted us to develop a paratransgenic strategy to control vectorial transmission of T. cruzi.
Here, the potential role of recombinant endoglucanases as anti-trypanosomal agents for paratransgenic application is examined. The surface of T. cruzi is covered by a thick coat of mucin-like glycoproteins that have been proposed to play a role in the binding of T. cruzi to the membrane surface of the vector gut.
We hypothesize that disruption of these glycoconjugates could arrest parasite development in the vector and abort the transmission cycle. In this work, we examine the effects of recombinant Arthrobacter luteus beta-1, 3-glucanase expressed via Rhodococcus rhodnii on T...
...Conclusions: These results suggest that recombinant beta-glucanase could be a powerful addition to the arsenal of effector molecules for paratransgenic control of Chagas disease. In future studies, the ability of beta-glucanase to function in combination with other effector molecules will be explored.
Dual targeting of T. cruzi should not only slow resistance but also permit synergistic or additive lethal effects on T.
cruzi.
Author: Christo JoseNicole KleinSarah WyssAnnabeth FieckIvy HurwitzRavi Durvasula
Credits/Source: Parasites &Vectors 2013, 6:65
Recombinant Arthrobacter beta-1, 3-glucanase as a potential effector molecule for paratransgenic control of Chagas disease
Chagas disease is most often transmitted to humans by Trypanosoma cruzi infected triatomine bugs, and remains a significant cause of morbidity and mortality in Central and South America. Control of Chagas disease has relied mainly on vector eradication.
However, development of insect resistance has prompted us to develop a paratransgenic strategy to control vectorial transmission of T. cruzi.
Here, the potential role of recombinant endoglucanases as anti-trypanosomal agents for paratransgenic application is examined. The surface of T. cruzi is covered by a thick coat of mucin-like glycoproteins that have been proposed to play a role in the binding of T. cruzi to the membrane surface of the vector gut.
We hypothesize that disruption of these glycoconjugates could arrest parasite development in the vector and abort the transmission cycle. In this work, we examine the effects of recombinant Arthrobacter luteus beta-1, 3-glucanase expressed via Rhodococcus rhodnii on T...
...Conclusions: These results suggest that recombinant beta-glucanase could be a powerful addition to the arsenal of effector molecules for paratransgenic control of Chagas disease. In future studies, the ability of beta-glucanase to function in combination with other effector molecules will be explored.
Dual targeting of T. cruzi should not only slow resistance but also permit synergistic or additive lethal effects on T.
cruzi.
Author: Christo JoseNicole KleinSarah WyssAnnabeth FieckIvy HurwitzRavi Durvasula
Credits/Source: Parasites &Vectors 2013, 6:65