tetano
Editor, Senior Moderator
J Virol. 2017 Jan 18. pii: JVI.02462-16. doi: 10.1128/JVI.02462-16. [Epub ahead of print]
[h=1]Recombinant adenoviruses displaying matrix 2 ectodomain epitopes (M2e) on their fiber proteins as universal influenza vaccines.[/h] Tang X[SUP]1[/SUP], Yang Y[SUP]1[/SUP], Xia X[SUP]1[/SUP], Zhang C[SUP]1[/SUP], Yang X[SUP]1[/SUP], Song Y[SUP]1[/SUP], Dai X[SUP]1[/SUP], Wang M[SUP]2[/SUP], Zhou D[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a zoonotic disease that poses severe threats to public health and the global economy. Re-emerging influenza pandemics highlight the demand for universal influenza vaccines. We developed a novel virus platform, AdC68-F3M2e, by introducing three conserved M2e epitopes into the HI loop of the chimpanzee adenovirus (AdV) fiber protein. The M2e epitopes were expressed sufficiently on the AdV virion surface without affecting fiber trimerization. Additionally, one recombinant adenovirus, AdC68-F3M2e(H1-H5-H7), induced robust M2e-specific antibody responses in BALB/c mice after two sequential vaccinations and conferred efficient protection against homologous and heterologous IV challenges. We find that AdV with tandem M2e epitopes in fiber is a potential strategy for IV prevention.
[h=4]IMPORTANCE:[/h] Influenza epidemics and pandemics severely threaten public health. Universal influenza vaccines have increasingly attracted interest in recent years. Here, we describe a new strategy that incorporates triple M2e epitopes into the fiber protein of chimpanzee adenovirus 68. We optimized the process of inserting foreign genes into the AdC68 structural protein by one-step isothermal assembly, and demonstrated that this 225bp HI loop insertion could be well tolerated. Furthermore, two doses of adjuvant-free fiber-modified AdC68 could confer sufficient protection against homologous and heterologous influenza infections in mice. Our results show that AdC68-F3M2e could be pursued as a novel universal influenza vaccine.
Copyright ? 2017 American Society for Microbiology.
PMID: 28100621 DOI: 10.1128/JVI.02462-16
[PubMed - as supplied by publisher]
[h=1]Recombinant adenoviruses displaying matrix 2 ectodomain epitopes (M2e) on their fiber proteins as universal influenza vaccines.[/h] Tang X[SUP]1[/SUP], Yang Y[SUP]1[/SUP], Xia X[SUP]1[/SUP], Zhang C[SUP]1[/SUP], Yang X[SUP]1[/SUP], Song Y[SUP]1[/SUP], Dai X[SUP]1[/SUP], Wang M[SUP]2[/SUP], Zhou D[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a zoonotic disease that poses severe threats to public health and the global economy. Re-emerging influenza pandemics highlight the demand for universal influenza vaccines. We developed a novel virus platform, AdC68-F3M2e, by introducing three conserved M2e epitopes into the HI loop of the chimpanzee adenovirus (AdV) fiber protein. The M2e epitopes were expressed sufficiently on the AdV virion surface without affecting fiber trimerization. Additionally, one recombinant adenovirus, AdC68-F3M2e(H1-H5-H7), induced robust M2e-specific antibody responses in BALB/c mice after two sequential vaccinations and conferred efficient protection against homologous and heterologous IV challenges. We find that AdV with tandem M2e epitopes in fiber is a potential strategy for IV prevention.
[h=4]IMPORTANCE:[/h] Influenza epidemics and pandemics severely threaten public health. Universal influenza vaccines have increasingly attracted interest in recent years. Here, we describe a new strategy that incorporates triple M2e epitopes into the fiber protein of chimpanzee adenovirus 68. We optimized the process of inserting foreign genes into the AdC68 structural protein by one-step isothermal assembly, and demonstrated that this 225bp HI loop insertion could be well tolerated. Furthermore, two doses of adjuvant-free fiber-modified AdC68 could confer sufficient protection against homologous and heterologous influenza infections in mice. Our results show that AdC68-F3M2e could be pursued as a novel universal influenza vaccine.
Copyright ? 2017 American Society for Microbiology.
PMID: 28100621 DOI: 10.1128/JVI.02462-16
[PubMed - as supplied by publisher]