tetano
Editor, Senior Moderator
J Clin Microbiol. 2015 Sep 16. pii: JCM.02165-15. [Epub ahead of print]
[h=1]Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse-Transcription Loop-Mediated Isothermal Amplification Methods.[/h] Bao H[SUP]1[/SUP], Feng X[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Shi J[SUP]1[/SUP], Zhao Y[SUP]1[/SUP], Gu L[SUP]1[/SUP], Wang X[SUP]2[/SUP], Chen H[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We developed hemagglutinin- and neuraminidase-specific one-step reverse-transcription loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and it did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity. The assays can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26378283 [PubMed - as supplied by publisher]
[h=1]Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse-Transcription Loop-Mediated Isothermal Amplification Methods.[/h] Bao H[SUP]1[/SUP], Feng X[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Shi J[SUP]1[/SUP], Zhao Y[SUP]1[/SUP], Gu L[SUP]1[/SUP], Wang X[SUP]2[/SUP], Chen H[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We developed hemagglutinin- and neuraminidase-specific one-step reverse-transcription loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and it did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity. The assays can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26378283 [PubMed - as supplied by publisher]