tetano
Editor, Senior Moderator
Biol Pharm Bull. 2018 Sep 20. doi: 10.1248/bpb.b18-00468. [Epub ahead of print]
[h=1]Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification.[/h] Ma S[SUP]1[/SUP], Li X[SUP]1[/SUP], Peng B[SUP]2[/SUP], Wu W[SUP]2[/SUP], Wang X[SUP]2[/SUP], Liu H[SUP]2[/SUP], Yuan L[SUP]1[/SUP], Fang S[SUP]2[/SUP], Lu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time reverse transcription polymerase chain reaction (RT-PCR) assay. The results data demonstrated that our method performed well and could can be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.
[h=4]KEYWORDS:[/h] Avian influenza A virus; H7N9; Lateral flow dipstick; Recombinase polymerase amplification
PMID: 30232304 DOI: 10.1248/bpb.b18-00468
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[h=1]Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification.[/h] Ma S[SUP]1[/SUP], Li X[SUP]1[/SUP], Peng B[SUP]2[/SUP], Wu W[SUP]2[/SUP], Wang X[SUP]2[/SUP], Liu H[SUP]2[/SUP], Yuan L[SUP]1[/SUP], Fang S[SUP]2[/SUP], Lu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time reverse transcription polymerase chain reaction (RT-PCR) assay. The results data demonstrated that our method performed well and could can be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.
[h=4]KEYWORDS:[/h] Avian influenza A virus; H7N9; Lateral flow dipstick; Recombinase polymerase amplification
PMID: 30232304 DOI: 10.1248/bpb.b18-00468
Free full text