tetano
Editor, Senior Moderator
Biosens Bioelectron. 2017 Apr 2;94:677-685. doi: 10.1016/j.bios.2017.03.068. [Epub ahead of print]
[h=1]Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye.[/h] Yeo SJ[SUP]1[/SUP], Cuc BT[SUP]1[/SUP], Kim SA[SUP]2[/SUP], Kim DT[SUP]1[/SUP], Bao DT[SUP]1[/SUP], Tien TT[SUP]1[/SUP], Anh NT[SUP]1[/SUP], Choi DY[SUP]3[/SUP], Chong CK[SUP]4[/SUP], Kim HS[SUP]5[/SUP], Park H[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza A virus; Novel pyridine-styrylpyridine fluorescent dye; Photostability; Rapid fluorescent immunochromatographic test
PMID: 28390319 DOI: 10.1016/j.bios.2017.03.068
[h=1]Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye.[/h] Yeo SJ[SUP]1[/SUP], Cuc BT[SUP]1[/SUP], Kim SA[SUP]2[/SUP], Kim DT[SUP]1[/SUP], Bao DT[SUP]1[/SUP], Tien TT[SUP]1[/SUP], Anh NT[SUP]1[/SUP], Choi DY[SUP]3[/SUP], Chong CK[SUP]4[/SUP], Kim HS[SUP]5[/SUP], Park H[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza A virus; Novel pyridine-styrylpyridine fluorescent dye; Photostability; Rapid fluorescent immunochromatographic test
PMID: 28390319 DOI: 10.1016/j.bios.2017.03.068