tetano
Editor, Senior Moderator
Biosens Bioelectron. 2017 Sep 18;100:348-354. doi: 10.1016/j.bios.2017.08.048. [Epub ahead of print]
[h=1]Rapid detection and subtyping of multiple influenza viruses on a microfluidic chip integrated with controllable micro-magnetic field.[/h] Zhang RQ[SUP]1[/SUP], Hong SL[SUP]1[/SUP], Wen CY[SUP]1[/SUP], Pang DW[SUP]1[/SUP], Zhang ZL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza viruses have threatened animals and public health systems continuously. Moreover, there are many subtypes of influenza viruses, which have brought great difficulties to the classification of influenza viruses during any influenza outbreak. So it is crucial to develop a rapid and accurate method for detecting and subtyping influenza viruses. In this work, we reported a rapid method for simultaneously detecting and subtyping multiple influenza viruses (H1N1, H3N2 and H9N2) based on nucleic acid hybridization on a microfluidic chip integrated with controllable micro-magnetic field. H1N1, H3N2 and H9N2 could be simultaneously detected in 80min with detection limits about 0.21nM, 0.16nM, 0.12nM in order. Moreover, the sample and reagent consumption was as low as only 3μL. The results indicated that this approach possessed fast analysis and high specificity. Therefore, it is expected to be used to simultaneously subtype and detect multiple targets, and may provide a powerful technique platform for the rapid detection and subtyping analysis of influenza viruses.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] DNA; Influenza virus; Magnetic bead; Microfluidic chip; SA-QDs
PMID: 28946106 DOI: 10.1016/j.bios.2017.08.048
[h=1]Rapid detection and subtyping of multiple influenza viruses on a microfluidic chip integrated with controllable micro-magnetic field.[/h] Zhang RQ[SUP]1[/SUP], Hong SL[SUP]1[/SUP], Wen CY[SUP]1[/SUP], Pang DW[SUP]1[/SUP], Zhang ZL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza viruses have threatened animals and public health systems continuously. Moreover, there are many subtypes of influenza viruses, which have brought great difficulties to the classification of influenza viruses during any influenza outbreak. So it is crucial to develop a rapid and accurate method for detecting and subtyping influenza viruses. In this work, we reported a rapid method for simultaneously detecting and subtyping multiple influenza viruses (H1N1, H3N2 and H9N2) based on nucleic acid hybridization on a microfluidic chip integrated with controllable micro-magnetic field. H1N1, H3N2 and H9N2 could be simultaneously detected in 80min with detection limits about 0.21nM, 0.16nM, 0.12nM in order. Moreover, the sample and reagent consumption was as low as only 3μL. The results indicated that this approach possessed fast analysis and high specificity. Therefore, it is expected to be used to simultaneously subtype and detect multiple targets, and may provide a powerful technique platform for the rapid detection and subtyping analysis of influenza viruses.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] DNA; Influenza virus; Magnetic bead; Microfluidic chip; SA-QDs
PMID: 28946106 DOI: 10.1016/j.bios.2017.08.048